[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material: Bis[4-(5-chloro-2-hydroxyphenylazo)-3-methyl-1-phenyl-5-pyrazolate] Ferrate, hydrogen
- Substance type: toner additive; charge control agent
- Physical state: solid
- Analytical purity: 98.02% (HPLC)
- Impurities (identity and concentrations): unknown: 0.98% (HPLC); water: 1.0%
- Composition of test material, percentage of components: ---
- Isomers composition: ---
- Purity test date: 5-Oct-07
- Lot/batch No.: 002-2
- Expiration date of the lot/batch: 2-Oct-09
- Stability under test conditions: stable
- Storage condition of test material: dry, cool; under exclusion of light
- Other: ---

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

not specified

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg test item was transferred to 1 l dilution water and stirred vigorously in a flask of adequate volume for 48 h
- Eluate: Filtration of test item particles to separate the eluate (0.22 µm filter; Millipore MCE-MF, cellulose nitrate and acetyl cellulose, no. GSWP04700)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: clone V
- Source: German Federal Environment Agency
- Age at study initiation (mean and range, SD): adult Daphnia incl 1 additional week of separate housing and feeding
- Feeding during test: not fed during test

ACCLIMATION
- Acclimation period: 1 wk
- Acclimation conditions (same as test or not): no
- Type and amount of food: algal suspension (Desmodesmus subspicatus and LiquiZellR
- Feeding frequency: daily
- Health during acclimation (any mortality observed): no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

Reconstituted water according to ISO 6341; characterisation: 294 mg/l CaCl2*2H2O; 123.25 mg/l MgSO4*7H2O; 64.75 mg/l NaHCO3; 5.75 mg/l KCl

Test temperature:

Room temperature (19.9-20.1°C)

pH:

8.0-8.5

Dissolved oxygen:

8.0-8.3 mg/l

Salinity:

see hardness

Nominal and measured concentrations:

nominal: 100 mg/l; measured: 17.3 µg/l

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 17.3 µg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The NOEC is >= 100 mg/l nominal loading, corresponding to a geometric mean measured analytical concentration of > 17.3 µg/l. The EC50 is assumed to be > 100 mg/l (nominal) or > 17.3 µg/l (analytical).

Executive summary:

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