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EC number: 601-409-2 | CAS number: 116020-44-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- Dec 18, 2000 - Mar 09, 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study was performed for a structural similar analoge of the compound. For this analogue, a chromosomal abberration assay was conducted according to Good Laboratory Practice (GLP). The study procedures described in this report are based on the requirements of the following guidelines:- OECD Guideline for the testing of Chemicals: Genetic Toxicology: 473 In vitro mammalian chromosome aberration test.- Japanase Testing Guidelines The test material shows a high similarity in chemical descriptors and physicochemical profile (logP, water solubility) to the query compound providing a sound basis for read across for this in vitro assay.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Deviations:
- no
- Principles of method if other than guideline:
- none
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 129738-34-7
- Cas Number:
- 129738-34-7
- IUPAC Name:
- 129738-34-7
- Test material form:
- other: solid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 after induction using Aroclor 1254
- Test concentrations with justification for top dose:
- 0, 10.2, 20.5, 40.9, 81.9, 164, 328, 655, 1310, and 2620 µg / ml medium; Precipitation of the test material was observed at concentrations larger or equal to 81.9 µg/mL.
- Vehicle / solvent:
- DMSO
Controlsopen allclose all
- Positive controls:
- yes
- Positive control substance:
- cyclophosphamide
- Remarks:
- Migrated to IUCLID6: +S9
- Positive controls:
- yes
- Positive control substance:
- mitomycin C
- Remarks:
- Migrated to IUCLID6: -S9
- Details on test system and experimental conditions:
- According to guideline
- Evaluation criteria:
- According to guideline
- Statistics:
- Descriptive statistics
Results and discussion
Test results
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Precipition: >=81.9 µg/mL
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):negative with and without S9Treatment of V79 cell cultures with the test material, in the absence and presence of S9 mix, did not increase the proportion of cells with aberrant chromosomes. Thus, the test material was not clastogenic in this in vitro test system.
- Executive summary:
Purpose
The purpose of the in vitro chromosome aberration test is to identify agents that cause structural chromosome aberrations in cultured mammalian cells thus providing information on possible health hazards for the test material and serve as a rational basis for risk assessment to the genotoxic potential of the test item in man.
Study Design
In this study the clastogenic potential of the test material was evaluated by examining its effects on the chromosomes of CHO cells, cultured in vitro and treated in the absence and presence of a rat liver metabolising system (S-9). The test methodology in this study is in accordance with current literature and complies with the OECD Guideline 473 (1997).
Results
Precipitation of the test material was observed at concentrations larger or equal to 81.9 µg/mL. The test material did not induce structural chromosome aberrations when tested at, or very close to, its limit of cytotoxicity following 24 hour treatment in the absence and presence of S9. No treatment-related increase of endoreduplications or polyploid cells was observed.
i.e. neither structural nor numerical aberrations were detected.
Conclusion
In conclusion, treatment of V79 cell cultures with the test material, did not increase the proportion of cells with aberrant chromosomes, thus the test material was not clastogenic in this in vitro test system.
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