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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1978
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given: comparable to guidelines/standards

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
yes
Remarks:
reporting details
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl benzoylformate
EC Number:
239-263-3
EC Name:
Methyl benzoylformate
Cas Number:
15206-55-0
Molecular formula:
C9H8O3
IUPAC Name:
methyl oxo(phenyl)acetate
Details on test material:
- Physical state: liquid

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: mean: males: 200g; females: 168g

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
CMC (carboxymethyl cellulose)
Remarks:
0.5% aqueous CMC + 2-3 drops Cremophor EL
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 50 - 31,6%

MAXIMUM DOSE VOLUME APPLIED: 20 mL/kg

Doses:
10000, 6810, 4640, 3160 mg/kg bw
No. of animals per sex per dose:
5 per sex per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: observations were done daily; weighing was done on day 4, 7 and 12
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, histopathology

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
6 800 - 10 000 mg/kg bw
Based on:
test mat.
Mortality:
At 10000 mg/kg bw day: Within 48 hours all male rats died. 7 days after application 4 of 5 female rats were dead. For details see table 1.
Clinical signs:
other: Highest Dose (10000 mg/kg): All animals showed in the highest dose apathy and staggering until day two after application. Prone/lateral position was seen until 2 hours after application of test substance. 2 hours after application animals showed atonia, n
Gross pathology:
All animals which were sacrificed scheduled showed normal organs without abnormality.
In the animals found dead (higest dose): the heart showed an acute dilatation and hyperemia. In the liver a centrilobular lobe outline is seen. The lungs of 2 rats showed slight edema.

Any other information on results incl. tables

Table 1: Mortality:

       died within            
 Dose [mg/kg]  conc [%]  animal #  1h 24h  48 h   7 d  14 d
 10000     50     5 male

 0/5

 4/5   5/5  5/5  5/5
 5 female  0/5  2/5  3/5  4/5  4/5
 6810     50     5 male  0/5 0/5  0/5  0/5  0/5 
 5 female  0/5  0/5 0/5   0/5  0/5
 4640     46,4     5 male  0/5 0/5  0/5   0/5 0/5 
 5 female  0/5 0/5  0/5  0/5  0/5 
 3160     31,6     5 male  0/5 0/5  0/5  0/5  0/5 
 5 female  0/5  0/5 0/5  0/5  0/5 

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information