[No public or meaningful name is available]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 05 JUL 2022 to 07 JUL 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Solvent for stock solution: Demineralized water.

Test organisms (species):

Daphnia magna

Details on test organisms:

Strain: STRAUS / BERLIN
Taxonomic position: Arthropoda - Crustacea
Sex: female
Age: between 0 and 24 hours
Origin: Umweltbundesamt Berlin
In-house breeding since: 27. September 2007

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not stated

Test temperature:

19.7 – 20.5 °C

pH:

7.7 - 7.8 (48 h)

Dissolved oxygen:

8.0 - 8.7 mg/L (48 h)

Nominal and measured concentrations:

Nominal concentrations: control, 4.6, 10, 22, 46, 100 mg/L
Measured concentrations for the nominal concentration of 4.6 mg/L component 1+2: 4.93 (0 h), 4.96 (24 h), 4.89 (48 h) mg/L
Measured concentrations for the nominal concentraiton of 22 mg/L based on component 1+2: 30.19 (0 h), 36.80 (24 h), 30.17 (48 h) mg/L
Measured concentrations for the nominal concentraiton of 100 mg/L based on component 1+2: 130.33 (0 h), 147.11 (24 h), 144.21 (48 h) mg/L

Details on test conditions:

Source/preparation of dilution water: M4-medium in deionised water

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorphenol (C6H4Cl2O, CAS No. 591-35-5)

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

41.27 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

Immobilisation in the controls < 10 %

Results with reference substance (positive control):

48 h-EC50 against 3,5-Dichlorphenol was determined as 2.04 mg/L

Reported statistics and error estimates:

Regression (ELx)

The measured concentrations were not in a range of ± 20 % of the nominal concentration, the determination of the biological results was based on the geometric mean of the measured concentrations

Validity criteria fulfilled:

yes

Conclusions:

The following result was determined for the test item (species: Daphnia magna) based on the concentration of the active ingredient (a. s.): 48h-EC50 41.27 mg/L

Description of key information

The 48 hr EC50 for Daphnia magna exposed to the test item was 41.27 mg a.i./L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

41.27 mg/L