Lithium iodide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 22 June 2022 to 27 July 2022

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2022

Report date:

2022

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

Test item and reference item analysis certificates. A deviation was written for the storage of reference item. There is no impact on the study and on the acquired data. The data generated are scientifically acceptable and valid.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Lithium iodide

EC Number:

233-822-5

EC Name:

Lithium iodide

Cas Number:

10377-51-2

Molecular formula:

ILi

IUPAC Name:

lithium iodide

Test material form:

solid

Specific details on test material used for the study:

Batch number 1280322A-0915P

Sampling and analysis

Analytical monitoring:

yes

Remarks:

Analytical method validation of lithium iodide anhydrous in UP water, algae medium and ISO 6341 medium

Test solutions

Vehicle:

no

Details on test solutions:

The test solutions will be prepared by dilution in test medium of a primary stock solution.
The test solutions concentrations will be expressed in concentrations of test item and the endpoints will be calculated with measured concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Strauss

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

19.8 to 24.7

pH:

7.6 - 7 9

Dissolved oxygen:

0 H = 8.0 - 8.2
48H = 7.7 - 8.3

Nominal and measured concentrations:

C07 = 0 mg/ L test item
C08 = 0.0625 mg/ L test item
C09= 0.125 mg/ L test item
C10 = 0.250 mg/ L test item
C11 = 0.500 mg/ L test item
C12 = 1 mg/ L test item

Details on test conditions:

Based on the results of the non-GLP range-finder the test solutions were prepared in
ISO medium at the definitive theoretical concentrations.
A control was set up with the test medium only. There were 4 replicates for each treatment and for the
control. 5 daphnids were introduced in each replicate (each one containing 50 mL of test solution).
Organisms were maintained at temperature in the range of 18°C to 22°C and light intensity under 300
lux (according OECD 202) with a light:dark cycle of 16 hours light and 8 hours dark.
Daphnids were not fed during the test. The test vessels must not be aerated during the test. The test
will be carried out without adjustment of pH.
After 24 and 48 hours, the number of immobile daphnids in each replicate and abnormal appearance
and behaviour were recorded.
pH and dissolved oxygen were recorded at the beginning and the end of the test.

Reference substance (positive control):

yes

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

ca. 0.42 mg/L

95% CI:

>= 0.38 - <= 0.46

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 to survival and reproduction of Daphnia magna were investigated under laboratory conditions.

Basis for effect:

mortality

Remarks:

The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 to survival and reproduction of Daphnia magna were investigated under laboratory conditions.

Effect concentrations 2

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 0.67 mg/L

95% CI:

>= 0.58 - <= 0.76

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 to survival and reproduction of Daphnia magna were investigated under laboratory conditions.

Basis for effect:

mortality

Remarks:

The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 to survival and reproduction of Daphnia magna were investigated under laboratory conditions.

Details on results:

The aim of this study was to assess the effects of the test item “Lithium Iodide Anhydrous” on the reproductive output of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).
Daphnia magna is a freshwater crustacean that is recommended by the OECD Guideline 202.
The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 to survival and reproduction of Daphnia magna were investigated under laboratory conditions.
Since the deviation between the measured concentrations and the nominal concentrations was withinthe range of ± 20 %, analysis of the results was based in the nominal concentrations.
The EC50 values were based on the nominal concentrations.

Results with reference substance (positive control):

Results with positive control are conformed.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The aim of this study was to assess the effects of the test item “Lithium Iodide Anhydrous” on the immobilisation output of Daphnia magna over a period of 48 hours under static conditions according to OECD Guideline 202 (April 2004).
Daphnia magna is a freshwater crustacean that is recommended by the OECD Guideline 202. The acute effects of “Lithium Iodide Anhydrous” at the definitive theoretical concentrations of 0; 0.0625; 0.125; 0.250; 0.500 and 1.0 mg test item.L-1 were investigated under laboratory conditions.

Since the deviation between the measured concentrations and the nominal concentrations was within the range of ± 20 %, analysis of the results was based in the nominal concentrations.

The EC50 values were based on the nominal concentrations:
EC50 - 24h = 0.67mg/L
EC50 - 48h = 0.42 mg/L