1,2-Benzenediamine, N1-methyl-4-(methylsulfonyl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2006

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

N1-methyl-4-(methylsulfonyl)benzene-1,2-diamine

EC Number:

615-907-2

Cas Number:

73097-51-5

Molecular formula:

C8H12N2O2S

IUPAC Name:

N1-methyl-4-(methylsulfonyl)benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

A dispersion of the test item with the loading rate of 100 mg/L was prepared by addition of 39.2 mg of the test item to 400 mL of test water. The test item was mixed into the test water using ultrasonic treatment for 15 minutes and intense stirring. The dispersion was stirred by a magnetic stirrer for 96 hours at room temperature in the dark to dissolve a maximum amount of the test item in the dispersion. After the stirring period, the dispersion was filtered through a membrane filter (pore size 0.45 IJm). The undiluted filtrate was used as highest concentrated test medium and as the stock solution for the preparation of the test media with lower test item concentrations by dilution with test water.

The undiluted filtrate (saturated solution containing the test item at the solubility limit of the test item in the test water) and the dilutions 1:10, 1:100 and 1:1000 of the filtrate were tested. In addition, a control (test water without test item) was tested in parallel.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20°C

pH:

7,8 - 7,9

Dissolved oxygen:

9,1 - 9,4 mg/l

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mobility

Remarks on result:

other: See "details on results"

Details on results:

In the undiluted filtrate (saturated solution containing the test item at the solubility limit of the test item in the test water), all daphnids were immobile after 24 hours of exposure. In the dilution 1:10 of the filtrate, 90% of the daphnids were immobile after 24 and 48 hours of exposure. In the dilutions 1:100 and 1:1000, no immobilization of daphnids was determined over the test period of 48 hours.

Any other information on results incl. tables

In the undiluted filtrate (saturated solution containing the test item at the solubility limit of the test item in the test water), all daphnids were immobile after 24 hours of exposure. In the dilution 1:10 of the filtrate, 90% of the daphnids were immobile after 24 and 48 hours of exposure. In the dilutions 1:100 and 1:1000, no immobilization of daphnids was determined over the test period of 48 hours.

Applicant's summary and conclusion

Conclusions:

Assuming that the solution is saturated at a concentration of 0.1 g/L, a dilution of 1:10 would result in a test concentration of 10 mg/L (no mobility after 48h: 90%) and with a dilution of 1:100 a concentration of 1 mg/L (no mobilityafter 48h: 0%). The EC50 would therefore be between > 1 to ≤ 10 mg/L and assuming that the substance is not rapidly degradable (worst case) this results in classification of the substance as Aquatic Chronic Category 2.