3-methoxy-N,N-dimethylpropan-1-amine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(3-methoxypropyl)dimethylamine

EC Number:

827-772-9

Cas Number:

20650-07-1

Molecular formula:

C6H15NO

IUPAC Name:

(3-methoxypropyl)dimethylamine

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

A fresh sample of activated sludge was collected from the aeration tank of the Zhu Yuan Domestic Wastewater Treatment Plant in Shanghai which treats predominantly domestic sewage with Anaerobic-Oxic process(A/O). The activated sludge was kept by aeration until used. The sludge was washed with mineral medium. After centrifugation, the supernatant was decanted. This procedure was repeated three times(4000 rpm, 4°C, centrifuge 20 minutes). Then, the concentrated sludge was weighed, dried at 105°C for 1 h. The final concentration of the activated sludge in the test medium was 30mg/L.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

22 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

without nitrification: ThOD(NH3) of DMMOPA = 2.32mg O2/mg.
Since the percentage biodegradation in the Day 28 was <10%, no correction for the oxygen consumed by nitrification of nitrite and nitrate was performed. ThODNH3 was used for calculation.

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

28 d

Remarks on result:

other: assuming no nitrification

BOD5 / COD results

Results with reference substance:

In the reference control, biodegradation of sodium benzoate reached 84% and 88% within 14 and 28 days of exposure, resp.
In the toxicity control with 22 mg/L DMMOPA, biodegradation of sodium benzoate reached 61% and 67% within 14 and 28 days of exposure, resp.
Therefore, the test substance was considered not to have a significant toxic or inhibitory effect on the activity of the microbial inoculum.

Any other information on results incl. tables

10-day window: not applicable as no noteworthy biodegradation took place.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Executive summary:

The test substance did not show noteworthy biodegradation (was not readily biodegradable) over 28 days in OECD 301F protocol.

As the reference substance sodium benzoate was readily biodegradable in the toxicity control experiment with 22 mg/L DMMOPA, DMMOPA did not have a significant toxic or inhibitory effect on the activity of the microbial inoculum.