Registration Dossier
Registration Dossier
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EC number: 276-696-7 | CAS number: 72490-01-8
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
Description of key information
Key value for chemical safety assessment
Additional information
Supporting studies
Acute oral toxicity in bees, Wilde 1982
A 24-hour acute oral toxicity test on honeybees (Apis mellifera) (10 bees/replicate/concentration) was conducted with the test substance at doses of 750, 1250, 1500 and 1750 ppm in sucrose solution, with solvent-control. The study was performed following 'Pesticides safety Precaution Scheme, Working Document D3, MAFF, UK (1979), and was not in compliance with GLP.At and above 1250 ppm, bees showed tumbling, excitement and ventral position within 1 hour after treatment. The24-Hour LD50 was estimated to be 1022 ppm in sucrose solution. The LD50 is equivalent to 20.4μg/bee, assuming that 20μL sucrose solution is consumed per bee.
EPA 141 -1, Winter 1990
A 48-hour acute contact toxicity test on honeybees (Apis mellifera) (2 replicates of 25 bees each per concentration) was conducted following the EPA 141 -1 guidance and in compliance with GLP. The bees were dosed with the test substance at doses of 13, 22, 36, 60 and 100 µg/bee, with solvent-control (acetone) and untreated control. Immediately following dosing, the bees were observed for mortality and signs of toxicity. Observations were made twice on the day of test initiation, and once each on Day 1 and Day 2 after dosing.
At test termination, mortality in the negative and solvent control groups was 2% and 42% respectively. The high mortality in the solvent control was found primarily in one replicate (72 %/48 h) and mainly approximately four hours after the completion dosing (60 %). The specific cause of this mortality could not be determined. Mortality in the 13 and 22 µg/bee doses was 8 and 6% respectively. One immobile bee was observed in the 22 µg/bee group approximately 10 minutes after dosing was completed. The immobility and mortality were not considered to be treatment related. All surviving honey bees were normal in appearance and behaviour throughout the test period. The mortality in the 36, 60 and 100 µg/bee dose groups was 14 %, 22 % and 28 % respectively. Immobile bees were observed in the 36 and 100 µg/bee groups approximately 10 minutes after dosing was completed and this was possibly treatment related in at least the 100 µg/bee dose. The mortality that occurred in these groups was dose responsive and considered to be treatment related. Based on the findings, the 48 -hour LD50 was estimated to be > 100 µg/bee.
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