Phenol, 2,6-di-tert-butyl-, 4-(trans-4-propylcyclohexyl)-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Sep 16, 2011 - Jun 18, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

17 July 1992

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The actual concentrations of the filtered test solution of nominal concentration of 100
mg/L test substance solution and blank control was measured at Oh, 96h and 24h, 48h,
72h (after and before the renewal) after the start of the test by a High Performance
Liquid Chromatography.

Vehicle:

no

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Common name: Zebra Fish
- Strain: Danio Rerio
- Source: Guangzhou Yang Shi Breeding FarmTEST ORGANISM
- Age at study initiation (mean and range, SD): -
- Length at study initiation (length definition, mean, range and SD): 26.04 mm ± 1.71 mm
- Weight at study initiation (mean and range, SD): 0.186 g±0.053 g


ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): same
- Type and amount of food during acclimation: standard food
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): daily


FEEDING DURING TEST
- Food type: no
- Amount: -
- Frequency: -

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

141 mg/L

Test temperature:

23 +/- 2 °C

pH:

8.18

Dissolved oxygen:

> 60 5 saturation

Details on test conditions:

14h light / 10 h dark

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (< 0.02023mg/L, LOD)

Details on results:

The 96h median lethal concentration (LC50) was greater than the saturation concentration of filtered test solution of nominal concentration of 100 mg/L test substance (< 0.02023mg/L, LOD).

Results with reference substance (positive control):

potassium dichromate: 24 h EC50 = 307 mg/L (SD: 273-346 mg/L)

Validity criteria fulfilled:

yes

Conclusions:

The informtion for this endpoint study record was obtained from an experimental study. The methods applied are fully compliant with OECD TG 203. The test was performed in a semi-static design. The chemical analysis was perfomed by means of HPLC. The 96h median lethal concentration (LC50) was greater than the saturation concentration of filtered test solution of nominal concentration of 100 mg/L test substance (< 0.02023mg/L, LOD).

Description of key information

OECD 203: LC50 (96 h) > 100 mg/L (nominal)

The 96h median lethal concentration (LC50) was greater than the saturation concentration of filtered test solution of nominal concentration of 100 mg/L test substance