Toluene-2-sulphonamide

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

23/06/1975 - 30/09/1975

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances", Japan

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

not specified

Radiolabelling:

not specified

Details on sampling:

- Sampling intervals/frequency for test organisms: Analysis of test fish was conducted four times at 2, 3, 4 and 6 weeks after the start of breeding; analysis were performed immediately after collection in 2 replicates.
- Sampling intervals/frequency for test medium samples: Twice per week, analytical samples were collected from each of the test water tanks in the first and second concentration areas, and quantitatively analyzed the test substances by a gas chromatograph (GC) method immediately after collection.
- Sample storage conditions before analysis: not applicable.
- Details on sampling and analysis of test organisms and test media samples (e.g. sample preparation, analytical methods):
- Pretreatment of water samples: extraction for GC analysis.
- Pretreatment of test organism samples: fish samples were crushed, treated with anhydrous sodium sulfate and chloroform and the filtrate was purified twice by column cromatographic method. The eluted fraction containing the test item was analysed by GC.

Vehicle:

no

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: Cyprinus carpio
- Source: not specified
- Length at study initiation (length definition, mean, range and SD): 10 cm on average
- Weight at study initiation (mean and range, SD): 30 g on average

Route of exposure:

aqueous

Test type:

not specified

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Total depuration duration:

0 wk

Test temperature:

25 ± 2 ºC

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 L glass covered glass tank.
- Type (delete if not applicable): closed.
- Material, size, headspace, fill volume: glass, size 100 L.
- No. of organisms per vessel: 15
- No. of vessels per concentration (replicates): 2

RANGE-FINDING / PRELIMINARY STUDY
- Test concentrations: not specified.
- Results used to determine the conditions for the definitive study: A preliminary study of the 48h-TLm of the test item on Rice fish (Oryzias latipes) was performed according to method JIS K0102. The 48h-TLm of the test item on fish was found to be 335 mg/L.

Nominal and measured concentrations:

Nominal concentrations: 3 mg/L in 1st concentration area, 0.3 mg/L in 2nd concentration area.

Reference substance (positive control):

not required

Lipid content:

4.3 %

Key result

Conc. / dose:

3 mg/L

Temp.:

25 °C

Type:

BCF

Value:

>= 0.4 - <= 0.9 L/kg

Basis:

not specified

Time of plateau:

6 wk

Calculation basis:

steady state

Key result

Conc. / dose:

0.3 mg/L

Temp.:

25 °C

Type:

BCF

Value:

>= 0.8 - <= 2.6 L/kg

Basis:

not specified

Time of plateau:

6 wk

Calculation basis:

steady state

Details on results:

- Mortality of test organisms: no.
- Behavioural abnormalities: not reported.
See any other details on results incl. tables.

Table 1. Bioaccumulation test results.

		2 W	3 W	4 W	6 W
1st Concentration area (3 ppm)	Test substance concentration (ppm)	3.2	3.4	3.4	3.4
	Bioconcentration factor (L/kg)	0.5	0.6	0.9	0.9
		0.6	0.4	0.6	0.8
2nd Concentration area (0.3 ppm)	Test substance concentration (ppm)	0.26	0.30	0.31	0.30
	Bioconcentration factor (L/kg)	*	0.8	**	2.6
		*	0.8	**	1.0

* No results were obtained, because all values of concentration in fish were under blank.

** Result was not determined, because analytical value in fish was trace.

Validity criteria fulfilled:

not specified

Conclusions:

Based on the study results, the test item has a BCF ≤ 2.6 L/kg in carp (worst case scenario).

Executive summary:

A bioaccumulation study of the test item in fish was performed according to Bioaccumulation test of a chemical substance in fish or shellfish (MITI No.1, 1974), equivalent to OECD 305C. Two levels of concentration as low as possible within the analytical limits (3 mg/L and 0.3 mg/L) were used, based on the results of the 48h-TLm determination of the test item in rice fish (Oryzias latipes), according to JIS K0102. The study was performed for 6 weeks on groups of Cyprinus carpio, along with periodical analytical determinations of the test item concentration in the tank water and in the test specimens by means of a validated analytical method. The BCF was determined at 2, 3, 4 and 6 weeks. The BCF values obtained ranged between 0.4 - 0.9 L/kg (3 mg/L) and 0.8 - 2.6 L/kg (0.3 mg/L). Therefore, the BCF of the test item is ≤ 2.6 L/kg in carp.

Description of key information

Key study. Method according to MITI No.1, equivalent to OECD 305C. The test item has a BCF ≤ 2.6 L/kg.

Key value for chemical safety assessment

BCF (aquatic species):

2.6 L/kg ww