Hexan-4-olide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

Dilution water

Details on test solutions:

Good quality reconstituted water was used. The water used in the test had hardness 86 mg, expressed as Ca content per liter (acceptance criteria 10< Ca < 250 mg), and pH7.71 (acceptance criteria 6 < pH <8.5). The reagents used for the preparation of reconstituted water were of analytical grade and the deionized water had a conductivity <=10 µS/cm.

Test organisms (species):

other: Brachydanio rerio

Details on test organisms:

As test system the fish species Brachydanio rerio was employed in the assay, as described by the guideline OECD 203:1992‘. The animals had a maximal length of 2.0 cm+-1.0 cm at the beginning of the test. they were in good health and free from any apparent malformation, as indicated in the veterinary Certificate.
For this test, the following fish batch was used: 02/2017.

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

86 mg

Test temperature:

22.5-23°C

pH:

7.33-7.77

Dissolved oxygen:

89.7%-90.6%

Conductivity:

<=10 uS/cm

Nominal and measured concentrations:

3.125 mg/I (nominal concentration)

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 3.125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mortality (fish)

Details on results:

Since at 96 hours no fishes e×posed to the test solution were dead, LC50 was evaluated to be > 3.125 mg/I (nominal concentration) or >3.093 mg/I (effective concentration) and the definitive test was not considered necessary.

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 203: 1992, under the test conditions applied, the LC50 of the test substance is calculated to be > 3.125 mg/I (nominal concentration)or > 3.093 mg/I (effective concentration).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

3.125 mg/L