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EC number: 271-434-8 | CAS number: 68555-58-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Dec. 2010 - Feb. 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 2008
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: from a wastewater treatment plant (ARA Ergolz II, Füllinsdorf, Switzerland) treating predominantly domestic wastewater
- Pretreatment: washed twice with tap water by centrifugation and the supernatant liquid phase was decanted. A homogenized aliquot of the final sludge suspension was weighed, thereafter dried and the ratio of wet to dry weight was calculated.
- Concentration of sludge: 4 g (±10%) dry material per liter - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Initial conc.:
- > 220 - < 222 other: mg O2/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: test water (according to guidelines)
- Solubilising agent: no
- Test temperature: 22 °C
- pH: 7.4 - 7.7
- pH adjusted: yes, for test water (7.8 to 7.4)
- Suspended solids concentration: final concentration of 30 mg dry material per liter
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL reaction vessels
- Number of culture flasks/concentration: 2
- Measuring equipment: The CO2 is adsorbed by soda lime, which results in a decrease of the total pressure in the airtight test flasks. The pressure drop is detected and converted into an electrical signal by means of an electrode type manometer.
SAMPLING
- Sampling frequency: at least on each working day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Procedure control: sodium benzoate
- Toxicity control: sodium benzoate and test item - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 86
- Sampling time:
- 28 d
- Details on results:
- After 7 days a degradation of 56 % was determined, after 14 days a degradation of 77 %, after 21 days a degradation of 84 % and after 28 days a degradation of 86 %. The 10 day window was passed. The degradation of the toxicity control was 78 % after 28 days. No toxicity of the test substance was observed in the toxicity control.
- Results with reference substance:
- In parallel preparations with the reference substance, sodium benzoate showed a degradation of 65 % was achieved within 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Within the test period of 28 days, a degradation of 86 % was determined for the test item.
- Executive summary:
The biodegradation of the test item was determined in a manometric respirometry test according to EU method C.4 D and OECD 301 F. The test substance is suspended in test medium, inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 22 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 86 % was determined. The degradation of the toxicity control was 78 % after 28 days. No toxicity of the test substance was observed in the toxicity control.
Reference
Description of key information
In a manometric respirometry test the biodegradation of the test item was determined to be 86 % after 28 days, therefore it was considered to be readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
The biodegradation of the test item was determined in a manometric respirometry test according to EU method C.4 D and OECD 301 F. The test substance is suspended in test medium, inoculated with activated sludge of a domestic WWTP and incubated for 28 days under aerobic conditions in the dark at 22 °C. During this period, the biodegradation of the test substance is determined on the basis of the generation of CO2. Within the test period of 28 days, a degradation of 86 % was determined. The degradation of the toxicity control was 78 % after 28 days. No toxicity of the test substance was observed in the toxicity control.
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