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EC number: 211-949-7 | CAS number: 719-59-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-amino-5-chlorobenzophenone
- EC Number:
- 211-949-7
- EC Name:
- 2-amino-5-chlorobenzophenone
- Cas Number:
- 719-59-5
- Molecular formula:
- C13H10ClNO
- IUPAC Name:
- (2-amino-5-chlorophenyl)(phenyl)methanone
- Test material form:
- solid: particulate/powder
Constituent 1
Method
- Target gene:
- TA1535: hisG46, uvrB, rfa
TA1537: his C3076, uvrB, rfa
TA100: hisG46, uvrBn rfa, R fact
TA98: his D3052, uvrb, rfa, R fact
WP2 uvrA: B/r/WP2
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Range finding : 10, 100, 500, 1000, 3000 μg/plate
Main study: 2, 10, 30, 60, 100, 200 μg/plate (without S9 mix)or 10, 30, 60, 100, 200 μg/plate (with S9 mix) - Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- N-ethyl-N-nitro-N-nitrosoguanidine
- other: 2-aminoanthracene ; 1-methyl-3-nitro-1-nitrosoguanidine
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Remarks on result:
- other: Cytotoxicity is starting generally at 100 μg/plate without S9 mix and at 200 μg/plate with S9 mix
Any other information on results incl. tables
Introduction of mutations in the absence of a metabolizing system (rat S9)
Experiment 1
Substance |
Conc. (μg/plate) |
Number of reverants per plate |
||||
TA 1535 |
TA 1537 |
TA 100 |
TA 98 |
WP2 uvrA |
||
Solvant control (DSO 100μl/pl.) |
|
11+-3 |
8+-3 |
131+-6 |
22+-13 |
21+-1 |
2-amino-5-chloro-benzophenone |
2 |
8+-4 |
7+-1 |
127+-21 |
20+-6 |
20+-6 |
|
10 |
6+-2 |
9+-3 |
128+-17 |
18+-1 |
20+-2 |
|
30 |
10+-1 |
9+-5 |
135+-26 |
18+-6 |
32+-1 |
|
60 |
9+-6 |
11+-6 |
110+-5 |
17+-1 |
23+-7 |
|
100P |
5+-2 |
10+-2 |
109+-16 |
19+-2 |
23+-5 |
|
200PP |
0 |
0 |
0 |
0 |
0 |
Control |
|
|
|
|
|
|
MNNG |
2 |
>2000 |
602 |
965 |
1551 |
528 |
9AA |
80 |
>2000 |
403 |
1243 |
1320 |
622 |
2 NF |
5 |
>2000 |
1554 |
1282 |
1541 |
633 |
ENNG |
2.5 |
|
720 |
1163 |
1471 |
594 |
Experiment 2
Substance |
Conc. (μg/plate) |
Number of reverants per plate |
||||
TA 1535 |
TA 1537 |
TA 100 |
TA 98 |
WP2 uvrA |
||
Solvant control (DSO 100μl/pl.) |
|
7+-1 |
6+-0 |
108+-16 |
13+-2 |
26+-9 |
2-amino-5-chloro-benzophenone |
2 |
11+-3 |
6+-1 |
114+-19 |
18+-5 |
28+-5 |
|
10 |
9+-3 |
6+-4 |
111+-13 |
17+-1 |
21+-7 |
|
30 |
7+-4 |
6+-2 |
125+-19 |
22+-2 |
18+-1 |
|
60 |
6+-4 |
6+-4 |
108+-7 |
17+-8 |
26+-2 |
|
100P |
9+-6 |
4+-1 |
87+-14 |
19+-9 |
23+-4 |
Control |
|
|
|
|
|
|
MNNG |
2 |
1019 |
857 |
1241 |
1165 |
544 |
9AA |
80 |
1537 |
519 |
1123 |
1389 |
452 |
2NF |
5 |
1522 |
867 |
1365 |
1252 |
542 |
ENNG |
2.5 |
1359 |
748 |
1243 |
1269 |
513 |
Induction of mutations in the presence of a metabolizing system (rat s9)
Experiment 1
Substance |
Conc. (μg/plate) |
Number of reverants per plate |
||||
TA 1535 |
TA 1537 |
TA 100 |
TA 98 |
WP2 uvrA |
||
Solvant control (DSO 100μl/pl.) |
|
8+-3 |
10+-4 |
112+-16 |
26+-2 |
25+-6 |
2-amino-5-chloro-benzophenone |
10 |
5+-2 |
8+-3 |
117+-11 |
25+-2 |
20+-7 |
|
30 |
4+-0 |
7+-4 |
124+-22 |
25+-2 |
25+-7 |
|
60 |
8+-3 |
10+-3 |
126+-10 |
21+-3 |
26+-4 |
|
100 |
6+-3 |
9+-3 |
131+-12 |
21+-5 |
18+-8 |
|
200P |
5+-3 |
7+-3 |
99+-15 |
25+-3 |
25+-3 |
Control |
|
|
|
|
|
|
2AA |
0.5 |
123 |
101 |
278 |
171 |
187 |
|
2.5 |
108 |
103 |
296 |
161 |
211 |
|
5 |
118 |
113 |
301 |
162 |
202 |
Induction of mutations in the presence of a metabolizing system (hamster s9)
Experiment 2
Substance |
Conc. (μg/plate) |
Number of reverants per plate |
||||
TA 1535 |
TA 1537 |
TA 100 |
TA 98 |
WP2 uvrA |
||
Solvant control (DSO 100μl/pl.) |
|
7+-2 |
12+-3 |
143+-15 |
28+-7 |
15+-7 |
2-amino-5-chloro-benzophenone |
10 |
9+-0 |
13+-2 |
119+-2 |
24+-3 |
19+-3 |
|
30 |
4+-2 |
9+-2 |
144+-8 |
24+-7 |
25+-2 |
|
60 |
6+-3 |
8+-2 |
135+-7 |
22+-4 |
27+-3 |
|
100 |
6+-2 |
6+-1 |
117+-14 |
20+-13 |
19+-6 |
|
200P |
7+-4 |
7+-2 |
92+-9 |
23+-3 |
21+-2 |
Control |
|
|
|
|
|
|
2AA |
0.5 |
214 |
174 |
>2000 |
>2000 |
670 |
|
2.5 |
245 |
217 |
>2000 |
>2000 |
697 |
|
5 |
250 |
201 |
>2000 |
>2000 |
694 |
Applicant's summary and conclusion
- Conclusions:
- None of the five tester strains showed increased mutagenie response, either in the absence (2-200μg/plate) or presence (10-200μg/plate) of S9 mix
In conclusion, it can be said that the investigation, under the experimental conditions described, gave no indication of any mutagenic activity of 2-amino-5-chlorobenzophenone in S.typhimurium strains TA1535, TA1537, TA100 and TA98 and E;coli WP2uvrA - Executive summary:
The mutagenic potential of 2-amino-5-ch1orobenzophenone, a degradation product of clorazepate dipotassium, was investigated in the bacteria strains Salmonella typhimurium TA1535, TA1537, TA100 and TA98 AND Escherichia coli WP2uvrA. The study was done using the plate incorporation assay with and without liver homogenate (S9 fraction, both from Aroclor 1254-pretreated male rats and from untreated mal hamsters) as metabolizing system. The concentrations of the test substance assayed had been determined in a pre-experiment and were selected on the strength of the precipitation on the plate and the bacteriotoxicity. In the main study, two independent experiments were carried out. Overall, varius concentrations of 2-amino-5chlorobenophenone ranging from 2 to 200μg/plate in the absence of the metabolizing system and from 10 to 200μg/plate in the presence of the metabolizing system were tested. 9-Aminoacridine, 2-aminoanthracene,1-ethyl-3-nitro-1-nitrosoguanidine, 1-methyl-3-nitro-1-nitrosoguanidine and 2-nitrofluorene served as positive controls to test the mutability of the bacterial strains as well as the activity of the metabolizing system. In the concentrations investigated, 2-amino-5-chlorobenzophenone did not show any mutagenic activity with or without the addition of S9 liver homogenate fractions. Bacteriotoxic effects were observed starting generally at 100μg/plate in the absence of, and at 200μg/plate in the presence of the metabolizing system. Precipitates in the agar were found starting at 100 and 200μg/plate, respectively. The known reversion properties were determined for the tester strains with the control substances; the positive responses confirmed the good metabolic activity of the liver homogenate fractions. It was concluded that the investigation, unde the experimental conditions described, gave no indication of any mutagenic activity of 2-amino-5-chlobenzophenone in S. typhimurium strains TA1535, TA1537, TA100 and TA98 and E.coli WP2uvrA.
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