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Diss Factsheets
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EC number: 201-182-6 | CAS number: 79-16-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (e.g. no analytical verification of the test concentrations)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: German Industrial Standard DIN 38412, part 15
- GLP compliance:
- no
Test material
- Reference substance name:
- N-methylacetamide
- EC Number:
- 201-182-6
- EC Name:
- N-methylacetamide
- Cas Number:
- 79-16-3
- Molecular formula:
- C3H7NO
- IUPAC Name:
- N-methylacetamide
- Details on test material:
- - test substance: N-Methylacetamide
- purity: 99 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- - test water: reconstituted freshwater was prepared from fully demineralized tap water, which was resalted by the addition of 294 mg/l CaCl2*2H2O, 123.3 mg/l MgSO4*7H2O, 64.8 mg/l NaHCO3, 5.8 mg/l KCl
- test water had a total hardness of 2.5 mmol/l, an acid capacity (to pH 4.3) of 0.8 mmol/l, 82 mg/l Ca, 12 mg/l Mg, > 8 mg/l oxygen, and ca. pH 8
Test organisms
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- - animal species: Leuciscus idus L., golden variety (golden orfe)
- mean body length: 5.3 cm (4.7-5.8 cm)
- mean body weight: 3.7 g (2.1-4.9 g)
- mean corpulence factor of the batch: 2.5
- duration of adaptation to test water and test temperature: 3 days
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
Test conditions
- Details on test conditions:
- - volume of test water: 10 l
- test vessels: all-glass aquaria (30x22x24 cm)
- aeration: continuously with oil-free air
- No. of animals per test concentration: 10
- loading (fish/vessel): 3.7 g/l
- temperature: 20 °C +- 1 °C
- nominal test concentrations: 0.0 (control), 1000, 2150, 4640, and 10000 mg/l
- observations: mortality after 1, 4, 24, 48, 72, and 96 h; symptoms after 4, 24 and 48 h
- measurements: pH and oxygen content after 1, 24, 48, 72, and 96 h
- the median lethal concentration (LC50) was calculated using Probit Analysis (Finney DJ, Probit Analysis, Cambr. Univ. Press, 3rd Edition, 1971)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3 390 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
- RS-Freetext:
Mortality:
----------
Nominal Cumulative mortality (n)
concentration (mg/l) 1 h 4 h 24 h 48 h 72 h 96 h
0 (Control) 0 0 0 0 0 0
1000 0 0 0 0 0 0
2150 0 0 1 3 3 4
4640 0 0 2 3 4 5
10000 0 0 7 9 10 10
Symptoms:
---------
Nominal Symptoms
concentration (mg/l) 1 h 4 h 24 h 48 h 72 h 96 h
0 (Control)
1000
2150 T
4640 T
10000 T N
Legend: T = tumbling; N = narcotic-like state
Oxygen:
-------
Nominal Oxygen content (mg/l)
concentration (mg/l) 0 h 24 h 48 h 72 h 96 h
0 (Control) 8.1 8.5 8.9 8.7 8.4
1000 8.2 8.6 8.7 8.7 8.6
2150 8.3 8.2 8.7 8.9 8.8
4640 8.0 8.0 8.9 8.9 8.7
10000 8.0 7.7 8.9 8.7 ---
pH:
---
Nominal pH
concentration (mg/l) 0 h 24 h 48 h 72 h 96 h
0 (Control) 8.0 7.8 8.0 8.7 8.4
1000 7.9 7.9 7.9 8.7 8.6
2150 7.8 7.8 7.9 8.9 8.8
4640 7.7 7.8 7.9 8.9 8.7
10000 7.7 7.7 8.0 8.7 ---
LC50
----
after 1 hour: > 10000 mg/l (1 % significance level)
after 4 hours: > 10000 mg/l (1 % significance level)
after 24 hours: 7240 mg/l (slope factor = 2.16)
after 48 hours: 4690 mg/l (slope factor = 2.21)
after 72 hours: 4600 mg/l
after 96 hours: 3390 mg/l (slope factor = 1.94)
NOEC: 1000 mg/l
LC0: 1000 mg/l
LC100: 10000 mg/l
The control with chloracetamide (48-h LC 50: ca. 26 mg/l)
corresponds to the normal sensitivity.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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