(S)-3-methyl-2-(2-oxotetrahydropyrimidine-1-yl)butyric acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July 5-9 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: done under GLP and OECD method

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

430-900-2

EC Name:

-

Cas Number:

192725-50-1

Molecular formula:

Hill formula: C9H16N2O3 CAS formula: C9H16N2O3

IUPAC Name:

3-methyl-2-(2-oxo-1,3-diazinan-1-yl)butanoic acid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At the beginning and then at 96hrs for the 22, 48 and 100 % concentrations. Also ran a stability sample concurrent to look for maintenace of test solution concentration.

Test solutions

Vehicle:

no

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: No auxiliary solvents were required.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebra fish: Brachydanio rerio (Cyprinidae species)

7 animals per concentration
weight = 0.27 g
lenght = 3cm

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Fish inspected at 24, 48, 72 and 96 hours

Test conditions

Hardness:

247 mg/L

Test temperature:

23 oC

pH:

7.8

Dissolved oxygen:

8.4 mg/l

Nominal and measured concentrations:

expected concentration = 100 mg/l @ 0 hours
Measured concentration = 106.91 mg/l @ 0 hours

expected concentration = 100 mg/l @ 24 hours
Measured concentration = 106.66 mg/l @ 24 hours
Nomin

Details on test conditions:

the fish are exposed to artificial lighting with a light period of 16 hours daily. fish are fasted during test.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The LC50at each observation (24,48, 72 and 96 hours) was higher than 100 mg/l.+
No animals of the control or treated group showed visible signs of abnormalities.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The EC50 for the test substance is estimated to be greater than 100 mg/l