Registration Dossier
Registration Dossier
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EC number: 430-900-2 | CAS number: 192725-50-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 Aug - 13 Aug, 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Done under GLP and OECD methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Version / remarks:
- Method C3, Directive 92/69/EEC
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 430-900-2
- EC Name:
- -
- Cas Number:
- 192725-50-1
- Molecular formula:
- Hill formula: C9H16N2O3 CAS formula: C9H16N2O3
- IUPAC Name:
- 3-methyl-2-(2-oxo-1,3-diazinan-1-yl)butanoic acid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Nominal test concentration: 100mg/L
Mean measured test concentrations at t=0: 103.18mg/L
Mean measured test concentrations at t=72hrs: 101.80mg/L
One control with algal growing medium was also used.
Cell concentrations were determined at 24, 48 and 72 hours after the start of the study.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Institute of FreshWater Ecology, Windermere Laboratory, Far Sawrey, Ambleside, Cumbria LA22 0LP, England.
Study design
- Test type:
- other: aeration
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 ± 2°C
- Nominal and measured concentrations:
- Nominal test concentration: 100mg/L
Mean measured test concentrations at t=0: 103.18mg/L
Mean measured test concentrations at t=72hrs: 101.80mg/L - Details on test conditions:
- Algae were cultured in double strength EPA algal assay medium for 72hrs under continuous uniform illumination of approx. 6,000 lux in the spectral range 400 -700 nm.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- EbC50(72h) : >100mg/L measured
ErC50(72h) : >100mg/L measured
NOEC b (72hr): >100mg/L measured
NOEC r (72hr): >100mg/L measured
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EbC50(72h) : >100mg/L measured
ErC50(72h) : >100mg/L measured
NOEC b (72hr): >100mg/L measured
NOEC r (72hr): >100mg/L measured
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