Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

15 August 2011 to 17 August 2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Deviations:

no

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: Guidance document on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23, December 14, 2000.

Deviations:

no

Principles of method if other than guideline:

not relevant

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

435-360-1

EC Name:

-

Cas Number:

144447-11-0

Molecular formula:

C20H22O5

IUPAC Name:

4-methoxyphenyl 4-[4-(oxiran-2-yl)butoxy]benzoate

Details on test material:

Identification: Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester
Chemical name: 4-methoxyphenyl-4(5,6-epoxyhexyloxy)benzoate
Molecular formula: C20H22O5
Molecular weight: 342.39
CAS Number: 144447-11-0
Description: White to off-white powder
Batch: 1001501002
Purity: 98.2%
Test substance storage: At room temperature in the dark
Stability under storage conditions: Stable
Expiry date: 01 February 2013

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Stability in water: Not indicated
Solubility in water: 0.586 mg/L (NOTOX project 497269)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples for possible analysis were taken from all test concentrations and the control according to the schedule below. In addition, filter containing undissolved residue was kept for possible analysis.

Frequency: at t=0 h and t=48 h
Volume: 2 ml from the approximate centre of the test vessels
Storage: Samples were stored in a freezer until analysis.

At the end of the exposure period, the replicates were pooled at each concentration before sampling.

Test solutions

Vehicle:

no

Details on test solutions:

The batch of Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester tested was a white to off-white powder with a purity of 98.2% and not completely soluble in test medium at the loading rate initially prepared.

Preparation of test solutions started with a loading rate of 100 mg/l applying a 2-day period of magnetic stirring to ensure maximum dissolution in test medium. The obtained mixture containing undissolved material was filtered through a 0.45 µm membrane filter (Whatman, RC55). The Water Soluble Fraction (WSF) was used as the highest test concentration. The lower test concentrations were prepared by subsequent dilutions of the WSF in test medium. The final test solutions were all clear and colourless.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820)
- Source: In-house laboratory culture with a known history.
- Age at study initiation: < 24 hours
- Method of breeding: With newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
- Feeding during test: No

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Remarks on exposure duration:

none

Post exposure observation period:

none

Test conditions

Hardness:

180 mg/l expressed as CaCO3

Test temperature:

19.2-20.9

pH:

7.9-8.0

Dissolved oxygen:

8.9-9.1

Salinity:

not measured

Nominal and measured concentrations:

Nominal :
Solutions containing 0.1, 1.0, 10 and 100% of the filtrate prepared at a loading rate of 100 mg/l.

Measured:


The measured concentration in the sample taken from the undiluted filtrate at the start of the test was 0.35 mg/l. During the exposure period the measured concentration had decreased to 0.26 mg/l
(75% of initial).

Based on these results, the geometric mean exposure concentration was calculated to be 0.30 mg/l.

Details on test conditions:

Test duration: 48 hours

Test type: Static

Test vessels: 100 ml, all-glass

Medium: Adjusted ISO medium

Number of daphnids: 20 each in the control and the highest concentration, 10 each in the remaining concentrations

Loading: 5 per vessel containing 80 ml of test solution

Light: 16 hours photoperiod daily

Feeding: No feeding

Aeration: No aeration of the test solutions.

Introduction of daphnids: Within 15 minutes after preparation of the test solutions.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 0.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: -

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: -

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.3 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: .

Details on results:

Behavioural abnormalities: no
- Immobility of control: no immobility was observed.
- Other adverse effects control: no
Behavioural abnormalities: no
- Immobility of control: no immobility was observed.
- Other adverse effects control: no

Results with reference substance (positive control):

The 24h-EC50 was 0.58 mg/l with a 95% confidence interval between 0.53 and 0.68 mg/l.

The 48h-EC50 was 0.41 mg/l with a 95% confidence interval between 0.36 and 0.46 mg/l.

Reported statistics and error estimates:

No EC50 could be calculated because the test substance proved to be non-toxic (EC50 > maximum soluble concentration tested).

Any other information on results incl. tables

Acute immobilisation of daphnids after 24 and 48 hours

Test substance^1 % WSF	Vessel number	Number Daphnia exposed	Response at 24 h		Response at 48 h
			number	Total %	number	Total %
control	A  B C D	5 5 5 5	0 0 0 0	0	0 0 0 0	0
0.1	A  B	5 5	0 1	10	0 1	10
1.0	A  B	5 5	0 0	0	0 0	0
10	A  B	5 5	0 0	0	0 0	0
100 (0.30)	A  B C D	5 5 5 5	0 0 0 0	0	0 0 1 0	5

1. Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester

() – between brackets the average exposure concentration is given [mg/l]

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Benzoic acid, 4-[4-(2-oxiranyl)butoxy]-, 4-methoxyphenyl ester did not induce acute immobilisation of Daphnia magna at a geometric mean concentration of 0.30 mg/l, i.e. an initially prepared loading rate of 100 mg/l after 48 hours of exposure (NOEC).

The 48h-EC50 was beyond the range tested, i.e. exceeded a geometric mean exposure concentration of 0.30 mg/l (an initially prepared loading rate of 100 mg/l).