Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment.

Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L

The LC 50 value for the test chemical was observed to be 24-h LC50 6.0 mg/L;48-h LC50 3.6–6.0 mg/L.

2.The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test.The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and itsstructurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 3.6-4.27   mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 3.6-4.27   mg/L .

Toxicity to aquatic algae and cyanobacteria:

Data available for the functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.The effect of test item was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 2.079mg/l.

2.The 72 h algal growth inhibition test with Raphidocelis subcapitata was done according to OECD 201 (2011) for the test chemical .

Algae were routinely cultured at 25±1 °C with light in Cyanobacteria BG-11 Freshwater Solution

The culture was split twice a week, adding fresh medium. Algae were exposed to the treatment in sterile 24-well plates with 2 mL of treatment solution to the respective microplate well, for three days.

The effect concentration of test material on green algae on Raphidocelis subcapitata for growth rate after 72 h was observed to be 1.20 mg/l.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and its functionally similar read across substance, it can be concluded that effect concetration value is in the range 1.120 -2.074 mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2 ".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 1.120 -2.074 mg/L .

Additional information

Short-term toxicity to aquatic invertebrate:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the short term toxicity of Daphnia magna of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2- (sulphonatooxy)ethyl]sulphonyl]phenyl]amino]- 1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]-4-hydroxy-5 -[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.Test material when used as a test material to evaluate its aquatic toxicity on Daphnia magna. The test animal was maintained. Twenty-four hours before the test, adult daphnids (gravid females) were sorted and the young one (neonates) produced from these adults were used in the experiment.

Initially, a 24-h preliminary test was carried out at exposure concentrations of 100, 10, and 1.0 mg/LThe selected concentration for the test was 100, 80, 60, 40, 20, and 10 mg/L

The LC 50 value for the test chemical was observed to be 24-h LC50 6.0 mg/L;48-h LC50 3.6–6.0 mg/L.

2.The acute aquatic toxicity was investigated with the 48 h daphnid immobilization assay for the test chemical. 24 h old neonatal Daphnia magna were used for the test.The effect concentration was observed to be 4.271 mg/l after 48 h and the confidence interval C.I. was 3.680-4.957.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and itsstructurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 3.6-4.27   mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 3.6-4.27   mg/L .

Toxicity to aquatic algae and cyanobacteria:

Data available for the functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical Tetrasodium 3-[[5-[[4-chloro-6-[[3-[[2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate (80019 -42 -7).The studies are as mentioned below:

1.The effect of test item was studied on the growth of fresh water green algaChlorella vulgaris.The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 2.079mg/l.

2.The 72 h algal growth inhibition test with Raphidocelis subcapitata was done according to OECD 201 (2011) for the test chemical .

Algae were routinely cultured at 25±1 °C with light in Cyanobacteria BG-11 Freshwater Solution

The culture was split twice a week, adding fresh medium. Algae were exposed to the treatment in sterile 24-well plates with 2 mL of treatment solution to the respective microplate well, for three days.

The effect concentration of test material on green algae on Raphidocelis subcapitata for growth rate after 72 h was observed to be 1.20 mg/l.

Thus, based on the above summarised studies,Tetrasodium 3-[[5-[[4-chloro-6-[[3-[ [2-(sulphonatooxy)ethyl]sulphony l]phenyl]amino]-1,3,5-triazin-2-yl]amino]-2-sulphonatophenyl]azo]- 4-hydrox y-5-[(1-oxopropyl)amino]naphthalene-2,7-disulphonate and its functionally similar read across substance, it can be concluded that effect concetration value is in the range 1.120 -2.074 mg/L. Thus, comparing this value with the criteria of CLP regulation, can be classified as "Aquatic chronic-2 ".Hence,based on the data available for the functionally similar read across, test chemical is likely to be toxic in the concentration range of 1.120 -2.074 mg/L .