(±)-neomenthol

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1985-05-10 - 1985-08-10

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

not specified

Radiolabelling:

no

Vehicle:

yes

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Lipid content in % : 4.7

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Nominal and measured concentrations:

nominal: 200 µg/l and 20 µg/l

Lipid content:

4.7 %

Time point:

end of exposure

Type:

BCF

Value:

>= 0.5 - <= 15 L/kg

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:200 µg/L

Type:

BCF

Value:

>= 4.6 - <= 11 L/kg

Calculation basis:

steady state

Remarks on result:

other: Conc.in environment / dose:20 µg/L

Validity criteria fulfilled:

not specified

Remarks:

expected to be valid source

Conclusions:

The bioconcentration factor ranges from <0.5 - 15 L/kg at 0.2 mg/L and <4.6-11L/kg at 0.02 mg/L test concentration using Cyprinus carpio as test organism.

Executive summary:

In a bioaccumulation study, corresponding to OECD Guideline 305C, a BCF of <0.5 - 15 L/kg at 0.2 mg/L test concentration and <4.6 - 11 L/g at 0.02 mg/L test concentration for menthol was determined with Oryzias latipes as test organism within 6 weeks.

Description of key information

The bioaccumulation of menthols in fish (Cyprinus carpio) was investigated in a test according to standard method from Japanese competent authority. Experimentally determined BCF values in the range of <0.5-15 L/kg indicate no significant bioaccumulation potential of menthols.

Key value for chemical safety assessment

BCF (aquatic species):

15 L/kg ww

Additional information

Since no data on the bioaccumulation propriety of the target substance are available, the potential for bioaccumulation is assessed on the basis of data from the structurally related menthol (stereo isomers). This read-across is in accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5. The target substance is a racemic mixture of D-, and L-menthol. This allows the use of experimental data from another isomeric mixture of D-menthol and L-menthol for the assessment of the target substance. A detailed category approach justification is provided in the technical dossier (see IUCLID Section 13).

The study with the source substance was conducted according to OECD 305C with freshwater fish (Cyprinus carpio). Fish were exposed to two different concentrations (0.2 and 0.02 mg/L) for up to 6 weeks. BCF values were determined in the range of 0.5 - 15 L/kg (0.2 mg/L) and 4.6 - 11 L/kg (0.02 mg/L). Both BCF values do not indicate a significant bioaccumulation potential. The slight variation of BCF values can be explained by the variation of the lipid content in fish (2-6%).

Based on the result from one structurally related source substance (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) it can be concluded that testing the target substance would result in comparable results.