9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium, salt with 4,5-dihydro-5-oxo-1-(4-sulphophenyl)-4-[(4-sulphophenyl)azo]-1H-pyrazole-3-carboxylic acid (3:1)

Endpoint summary

Description of key information

Toxicity to aquatic invertebrates:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted for 9-[2-(ethoxycarbonyl) -phenyl] -3,6-bis(ethylamino)-2,7-dimethylxanthylium.The EC50 value was estimated to be 117.563 mg/l when 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis (ethylamino) -2,7- dimethyl xanthylium exposed to daphnia magna for 48 hrs.

Short term toxicity to algae:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis (ethylamino) -2,7-dimethyl xanthylium (65138-66-1). The EC50 value was estimated to be 186.836 mg/l when 9-[2-(ethoxycarbonyl)phenyl]-3,6- bis(ethylamino)-2,7-dimethyl - xanthylium exposed to Desmodesmus subspicatus for 72hrs.

Additional information

Summarized result of toxicity of the chemical9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthyliumon the growth of aquatic invertebrates and algae was studied by considering and collecting the data from various databases for target chemical. Some RA (read across) chemicals was also studied because of supporting the classification of target9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthyliumchemical. The result are as mention:

Toxicity to aquatic invertebrates:

Summarized result of toxicity of the chemical 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium on the growth of aquatic invertebrates are as mention below:

In the first weight of evidence study from QSAR, 2017 for target chemical has been consider,based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the six closest read across substances, toxicity on daphnia magna was predicted for 9-[2-(ethoxycarbonyl) -phenyl] -3,6-bis(ethylamino) -2,7- dimethyl -xanthylium.The EC50 value was estimated to be 117.563 mg/l when 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis (ethylamino) -2,7- dimethyl xanthylium exposed to daphnia magna for 48 hrs.

 

In the second weight of evidence study for (518-47-8) from ABITEC report 2017, Determination of the inhibition of the mobility of daphnids was carried out with the substance Disodium 2-(3-oxo-6-oxidoxanthen-9-yl) benzoate according to OECD Guideline 202. A limit test at sample concentration of 100 mg/L was performed. Effects on immobilisation were observed for 48 hours. The median effective concentration (EC50) for the test substance, Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate, in Daphnia magna was determined to be >100 mg/L for immobilisation effects. Based on this EC50 value and after comparing with CLP criteria for aquatic classification of the substance it is concluded that the substance, Disodium 2-(3-oxo-6-oxidoxanthen-9-yl)benzoate does not exhibit short term toxicity to aquatic invertebrate (Daphnia Magna).

 

Similarly in the third weight of evidence study for same RA chemical 1995, Ecological assessments included both acute and chronic effects for the 3 life forms: fish, Cladocera (water flea, daphnia), and algae. The concern levels identified by use of the SAR technique are expressed ad HIGH, MODERATE, LOW. These values are toxicity values, not exposure values. SAR results are generally regarded by EPA as being reliable. Dyes with aquatic toxicity values below 1mg/l received a HIGH concern rating, those with toxicity values ranging from 1-100mg/l received a MODERATE concern rating and those with toxicity values greater than 100mg/l received a LOW concern rating. The Median Lethal Concentration which is estimated to be lethal to 50% of Cladocera (water flea) in 48 hours exposure to the test chemical (LC50) was 165mg/l. Since the LC50 for the test chemical was determined to be 165 mg/l, it falls in the low concern category in the aquatic toxicity ratings. Thus, based on the result according to the CLP regulation it was considered that the test chemical was not classified to short term aquatic invertebrates.

 

 

Similarly in the fourth weight of evidence study for another RA chemical (502-33-4) OECD SIDS 2004, Short term toxicity of Caprolactone on the growth of daphnia magna was studied by the exposure of chemical with the daphnia for 48 hrs. Test was performed according to the OECD guideline. Daphnia expose for 48 hrs under the proper conditions of temperature and ph.500.1 mg epsilon caprolactone was stirred in 500 ml M4 medium for about 10 minutes at 20 degrees C resulting in a stock solution of 1000 mg/l. Then different concentration and control was prepared. 0, 62.5, 125, 250, 500 and 1000 mg/l concentration was prepared for the study. After the exposure of 48 hrs. The various effect concentration was observed EC0 was 125 mg/l, EC50 was 204 mg/l and the EC100 was 500 mg/l. Based on the immobility of daphnia magna due to the exposure of chemical Caprolactone for 48 hrs, the EC50 was 204 mg/l. Thus the chemical was consider as non-toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

 

Thus based on the various studies it was concluded that the chemical 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium (65138-66-1) was consider as non-toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

Short term toxicity to algae:

Study was conducted for evaluatin the toxic nature of chemical 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium (65138-66-1). Summarized results are as follows:

In the first predicted report for target chemical from QSAR toolbox 2017 study was conducted on the toxicity of chemical on the growth of algae. Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the five closest read across substances, toxicity on Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) was predicted for 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium (65138-66-1). The EC50 value was estimated to be 186.836 mg/l when 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium exposed to Desmodesmus subspicatus for 72hrs.

 

In the second weight of evidence study for (518-47-8), UERL report. The effect of test item Disodium 2-(3-oxo-6-oxidoxanthen-9-yl) benzoate, was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be 209.24 mg/l. Thus, based on the EC50 value, test substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

Similarly in the third weight of evidence study for RA chemical (504-44-3). Short term toxicity of Caprolactone on the growth of algae Scenedesmus subspicatus was studied by the exposure of chemical with the daphnia for 48 hrs. The test was performed according the OECD guideline 201. Test conducted at the different nominal concentrations 0, 102, 256, 640, 1600, 4000 mg/l. Positive control i.e. potassium dichromate was used which shows biomass inhibition at the concentration of 0.47 mg/l. After the 72 hrs. Of exposure the NOEC was 256, LOEC observed at 640 mg/l, EC10 was 484 mg/l, EC50 1217 mg/l, EC90 was observed at > 4000 mg/l. Based on the inhibition of biomass of Scenedesmus subspicatus (Algae) due to the exposure of chemical Caprolactone, the EC50 was 1217 mg/l. Thus the chemical was consider as non-toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

 

 

Based on the various studies it was concluded that the chemical 9-[2-(ethoxycarbonyl)phenyl]-3,6-bis(ethylamino)-2,7-dimethylxanthylium (65138-66-1) was consider as non-toxic and can be consider to be not classified as toxic as per the CLP classification criteria.

thus based on the various studies for the toxicity of chemical on the growth of daphnia and algae, it was concluded that the chemical 9-[2-(ethoxycarbonyl)phenyl] -3,6 -bis(ethylamino)-2,7-dimethylxanthylium (65138-66-1) was consider as non-toxic and can be consider to be not classified as toxic as per the CLP classification criteria.