2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt

Endpoint summary

Description of key information

Biodegradation in water:screening tests

28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test chemical 2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt(CAS No. 12227-78-0). The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions.  This gave the bacterial count as 107to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.074%. The mean BOD values (mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 50.050 %after 7 days and 68.068 % after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical 2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt, CAS No. 12227-78-0 was observed to be 0.04 mgO2sup>/mg. ThOD was calculated as 0.653 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 6.126%.Based on the results, the test item, under the test conditions, was considered to be not biodegradable at 20 ± 1°C over a period of 28 days.

Additional information

Biodegradation in water:screening tests

28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test chemical 2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt(CAS No. 12227-78-0). The study was performed at a temperature of 20± 1°C. Mixture of domestic waste water, surface soil and soil samples was used as a test inoculum for the study. This inoculum was collected and were mixed to get diluted suspension. The inoculum was kept aerobic until being used for experiment by supplying organic and inorganic sources required by micro flora to sustain at controlled laboratory conditions.  This gave the bacterial count as 107to 108 CFU/ml. At the regular interval microbial plating was also performed on agar to confirm the vitality and CFU count of microorganism. OECD mineral medium was used for the study. The test system included control, test item and reference item. The concentration of test and reference item ( Sodium Benzoate) chosen for both the study was30 mg/L, while that of inoculum was 10 ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The % degradation of procedure control (Sodium Benzoate) was also calculated using BOD & ThOD and was determined to be 74.074%. The mean BOD values (mg O2/l) in control on 28th day was 9 mg O2/l, thereby fulfilling the control validity criteria (i.e., The oxygen consumption of the inoculum blank is normally 20-30 mg O2/l and should not be greater than 60 mg/l in 28 days). Degradation of Sodium Benzoate exceeds 50.050 %after 7 days and 68.068 % after 14 days. The activity of the inoculums is thus verified and the test can be considered as valid. The BOD28 value of test chemical 2-(3,6-dihydroxy-2,4,5,7-tetraiodoxanthen-9-yl)benzoic acid, aluminium salt, CAS No. 12227-78-0 was observed to be 0.04 mgO2sup>/mg. ThOD was calculated as 0.653 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was determined to be 6.126%.Based on the results, the test item, under the test conditions, was considered to be not biodegradable at 20 ± 1°C over a period of 28 days.