Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 282-468-8 | CAS number: 84229-70-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
Not readily or inherently biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Screening tests for biodegradation of Reactive Black 5 and Reactive Blue 203 in water showed that the test substance is not readily or inherently biodegradable. However, this reactive dye is rapidly removed from the dye-bath as shown by decolorisation of the test medium.
A study was conducted to assess the ready biodegradability of the Structural Analogue 01 according to EU Method C.4-D (which is in most parts equivalent to OECD Guideline 301 F), in compliance with GLP.
100 mg/L of the test substance was inoculated (in triplicate) with non-adapted activated sludge in a closed flask at a constant temperature (22±2°C) for up to 28 d. Degradation was assessed by the determination of Biochemical Oxygen Demand (BOD) on Days 2, 6, 8, 12, 14, 16, 20, 22, 26 and 28. A toxicity test and a control solution containing the reference substance benzoic acid, sodium salt (100 mg/L) together with inoculum were used for validation purposes. The test substance degraded 0% by Day 28. No toxicity of the test substance was observed in the toxicity control. The reference compound showed 90% degradation at Day 14.
Hence, under the study conditions, the test substance was considered to be ‘not readily biodegradable’.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
