Cobalt di(acetate)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline with acceptable restrictions: limited reporting

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Acute oral toxicity of inorganic cobalt compounds in rats.

Author:

Speijers GJA, Krajnc EI, Berkvens JM, van Logten MJ

Year:

1982

Bibliographic source:

Fd Chem Toxic 20: 311

Reference 2

Reference Type:

review article or handbook

Title:

Indirect food additives and polymers

Author:

Sheftel VO

Year:

2000

Bibliographic source:

Migration and Toxicology, Lewis Publishers, Boca Raton, Florida

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(10 day observation)

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

6147-53-1

EC Number:

612-153-6

Cas Number:

6147-53-1

IUPAC Name:

6147-53-1

Details on test material:

- Name of test material (as cited in study report): Cobalt(II) acetate tetrahydrate
- Molecular formula (if other than submission substance): Co(CH3COO)2 4H2O
- Molecular weight (if other than submission substance): 249.1 g/mol
- Physical state: solid
- Analytical purity: 99 %
- Other: Apart from chemically combined water the test substance is identical to CAS 71-48-7

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: animal breeding unit, National Institute of Public Health, Bilthoven, the Netherlands
- Weight at study initiation: approx. 100 g

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

250; 375; 560; 840; 1260 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 10 days
- Frequency of observations: rectal temperature: 1.5, 24 and 48 hours after treatment
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, other: rectal temperature

Statistics:

The oral LD50 values of male and female rats combined were calculated according to the method of 'maximum likelihood ' according to Finney (1971).

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

503 mg/kg bw

Remarks on result:

other: LD50 for anhydrous compound (calculated). LD50 for compound tested was 708 (569-880) mg/kg bw (95 % confidence interval).

Mortality:

Individual values not reported.

Clinical signs:

highest dose level: sedation, diarrhea;
respiratory disturbances
time- and dose-related drop in body temperature of 2.5 to 7.5°C

Gross pathology:

hemorrhages and distrophic changes in the liver, kidney and heart

Any other information on results incl. tables

LD50 calculated for Cobalt(II) ion = 168 mg/kg bw.