Iron trichloride

Specific investigations: other studies

Administrative data

Endpoint:

biochemical or cellular interactions

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: This information was obtained from the public IUCLID 2000 CD-ROM. The original report has not been reviewed further. Assessment of data quality to current OECD standards is not possible and the study has therefore been assigned Reliability 4.

Data source

Reference

Reference Type:

publication

Title:

Iron-induced DNA damage and synthesis in isolated rat liver nuclei.

Author:

Shires TK

Year:

1982

Bibliographic source:

PMID 7138506 Biochem J 205(2):321-9. http://www.biochemj.org/bj/205/0321/2050321.pdf

Materials and methods

Endpoint addressed:

genetic toxicity

Test material

Test material information

Constituent 1

Reference substance name:

Iron dichloride

EC Number:

231-843-4

EC Name:

Iron dichloride

Cas Number:

7758-94-3

Molecular formula:

FeCl2

IUPAC Name:

Iron (II) chloride

Results and discussion

Details on results:

Incubation of iron with isolated rat liver nuclei stimulated fragmentation of single-stranded DNA, incorporation of [3H]thymidine into DNA and the binding of 59Fe to DNA. FeCl2 was about twice as active as FeCl3. Lipid peroxidation took place in nuclei incubated with FeCl2, but not with FeCl3. Generation of reactive forms of oxygen was required for iron-mediated DNA damage, but evidence for direct interaction of reactive oxygen with DNA was not found. Apparent adducts of iron bound to DNA seemed to be formed by an enzymic mechanism.