N-(carboxymethyl)-N-(phosphonomethyl)glycine

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005.03.07-2005.03.11

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2005

Report date:

2005

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Parcom Protocols on Methods fir the Testing of Chemicals used in the Offshore Industry

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N-(carboxymethyl)-N-(phosphonomethyl)glycine

EC Number:

227-824-5

EC Name:

N-(carboxymethyl)-N-(phosphonomethyl)glycine

Cas Number:

5994-61-6

Molecular formula:

C5H10NO7P

IUPAC Name:

2-[(carboxymethyl)(phosphonomethyl)amino]acetic acid

Details on test material:

- Name of test material (as cited in study report): PMIE or Phosphonomethyliminodiacetic acid
- Substance type: Solid
- Physical state: White powder
- Analytical purity: 99 % w/w
- Purity test date: 15.06.2005
- Lot/batch No.: SB-7-4-89
- Expiration date of the lot/batch: 11.09.2005
- Stability under test conditions: Stable for 6 months
- Storage condition of test material: at - 20 ˚C

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 200 mg/L analysed (the highest exposure solution concentration)
- Sample storage conditions before analysis: at - 20 ˚C

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Stock solution: Nominiel concentration of the highest exposure solution: 200 mg/L, actual concentration 195 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): None

Test organisms

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

TEST ORGANISM
- Common name: Sheephead minnow
- Source: originally obtained from a stock culture at Opus Plus Ltd.(the former ERT Orkney water Technology Centre, and cultured at DHI since June 2003. Animals from this DHI culture was used for this study
- Length at study initiation (length definition, mean, range and SD):standard length 18.5 ± 2.37 cm
- Weight at study initiation (mean and range, SD): 0.17 ± 0.06 g
- Method of breeding: No data
- Feeding during test: No

ACCLIMATION
- Acclimation conditions : no acclimatation needed, test done under same conditions at same facility as test animals was optained from.

Study design

Test type:

static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

20 ± 0.5 ˚C

pH:

7.9-8.2

Dissolved oxygen:

94 -100 % saturated

Salinity:

32 ‰

Nominal and measured concentrations:

Nominial concentrations: 0 (control); 10; 20; 50; 100; 200 mg/L
Measured concentration: 195 mg/L measured for the highest nominial concentration, 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Type: open
- Material, size, headspace, fill volume: glass, 5 L, ?, 4 L
- Aeration: yes
- No. of organisms per vessel: 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from the North Sea, filtered through Millipore filters (10; 5; 0.5; 0.22 μm)
- Particulate matter: None
- Culture medium different from test medium: No


OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: light/dark 16:8 hours

TEST CONCENTRATIONS

- Test concentrations: 0 (control);10; 20; 50; 100 and 200 mg/L
- Results used to determine the conditions for the definitive study: yes

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol, CAS No. : 591-35-5, at 2.3 mg/L.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks:

No mortality or any other significant effects were recorded at even the highest concentration, 200 mg/L

Details on results:

- Behavioural abnormalities: None
- Observations on body length and weight: None
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: Not known

Results with reference substance (positive control):

- Results with reference substance valid? Yes
- Mortality: 20 % at 2.3 mg/L
- LC50: No calculated

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

Mortality in controls under 10 %, oxygen over 60%, mortality with reference test within acceptable range, 20-80 %

Conclusions:

LC50(96 h) of N-(carboxymethyl)-N-(phosphonomethyl)-glycine, CAS No. 5994-61-6, exceeds 200 mg/L for Cyprinodon variegatus