Isobutyryl chloride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report which meets basic scientific principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1969

Report date:

1969

Materials and methods

Principles of method if other than guideline:

other: BASF-test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyryl chloride

EC Number:

201-194-1

EC Name:

Isobutyryl chloride

Cas Number:

79-30-1

Molecular formula:

C4H7ClO

IUPAC Name:

2-methylpropanoyl chloride

Details on test material:

- Name of test material (as cited in study report): Isobuttersäurechlorid

Test animals

Species:

mouse

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Doses:

0.25, 0.64, 0.80, 1, 1.25, 1.6, 4, 32 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations: 1 hour, 1 day, 2 days, 7 days and 14 days after injection.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, gross pathology

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

0.8 - 1 mg/kg bw

Based on:

test mat.

Mortality:

After 14 days:
0/20 deaths at 0.25 mg/kg bw
6/20 deaths at 0.64 mg/kg bw
4/20 deaths at 0.8 mg/kg bw
12/20 deaths at 1 mg/kg bw
16/20 deaths at 1.25 mg/kg bw
19/20 deaths at 1.67 mg/kg bw
20/20 deaths at 4 and 32 mg/kg bw
At the highest dose first deaths were observed 1 hour after administration. Over the entire dose range first deaths occurred after about 48 hours.

Clinical signs:

0.25-1.6 mg/kg bw:
directly after injection: tremble, convulsions and uncoordinated movements, extension and retardation of extremities during movement, heavy breathing, avoidance of abdominal position and fallen-in flanks. The following days: abdominal posture, apathy, closed eyes with stuck together eyelids, apnea and bristly fur. Surviving animals showed no symptoms after 3-11 days.

4 and 32 mg/kg bw:
directly after injection: jumping and stagger, heavily accelarated breathing. After about 10 minutes: abdominal position, bushy fur, closed eyes and apathy. Continuation of symptoms untill death in the following days.

Gross pathology:

Animals dying during the test: dark-blooded abdominal cavity, corosive lesions of gastrointestinal tract, slight abdominal adhesions, fatty liver (1 naimal) vessel-injected winding of small intestine.
Animals sacrificed after the observation period: adhesions in the liver area