Ibuprofen

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ibuprofen

EC Number:

239-784-6

EC Name:

Ibuprofen

Cas Number:

15687-27-1

Molecular formula:

C13H18O2

IUPAC Name:

2-(4-isobutylphenyl)propanoic acid

Details on test material:

- Name of test material (as cited in study report): Ibuprofen 38
- Physical state: solid/white
- Analytical purity: 100%
- Batch No.: IB1Q0091

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared seperatly by directly adding test substance to 1 L of test medium and stirred for approximately one day. Undissolved test substance was removed by filtration with a membrane filter (pore width 0.2 μm).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory of Ecotoxicology of the Experimental Toxicology and Ecology of BASF SE in Ludwigshafen
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: none

CULTURE CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Culture media: synthetic fresh water (Elendt M4) prepared according to OECD 202
- Temperature: 20 ± 1 °C
- Diet: Unicellular algae Desmodesmus subspicatus, cultured continuously at the test facility
- Feeding: Daily
- Renewal of the culture water: Twice weekly

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.20 – 3.20 mmol/L

Test temperature:

19-21 °C

pH:

7.5 – 8.5

Dissolved oxygen:

8.3 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 4.6, 10, 22, 46, 100 mg/L
Mean measured concentrations: -, 2.8, 9.9, 22.1, 46.6, 101.2 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass beakers (nominal volume 100 ml)
- Test volume: 50 ml
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes. Composition according to OECD guideline 202.
- Alkalinity: 0.87 mmol/L
- Ca/Mg ratio: about 4:1
- Conductivity: 574 µS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature - continuous monitoring; pH and oxygen content - after 0 and 48 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: About 20 – 850 lux at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: immobilisation after 0, 24, 48 hours

TEST CONCENTRATIONS
- Prelimenary study: yes, the 48 hour EC50 was between 10 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

36.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none
- Effect concentrations exceeding solubility of substance in test medium: no remarkable observations, clear test medium

Results with reference substance (positive control):

Effect concentrations:
EC0 = 9.9 mg/L
EC50 = 36.9 mg/L
EC100 = 101.2 mg/L