Decyl methacrylate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Study well documented, meets generally accepted scientific principles, acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1987

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Principles of method if other than guideline:

Method: Ames test

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Decyl methacrylate

EC Number:

221-657-1

EC Name:

Decyl methacrylate

Cas Number:

3179-47-3

Molecular formula:

C14H26O2

IUPAC Name:

decyl 2-methylprop-2-enoate

Details on test material:

2-Propenoic acid, 2-methyl, decylester (CAS: 3179-47-3)
Supplier: Pfaltz & Bauer
Purity: no data, commercial grade assumed

Method

Target gene:

his-

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 mix from Aroclor 1254 induced Sprague Dawley rat liver and male Syrian Hamster liver.

Test concentrations with justification for top dose:

Dosis: 33 - 10000 µg/plate
At least five doses tested in triplicate.

Vehicle / solvent:

DMSO (Dimethyl sulfoxid)

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: without S9: sodium azide, 9-aminoacridine and 4-nitro-o-phenylenediamine. With S9: 2-aminoanthracene

Details on test system and experimental conditions:

Salmonella typhimurium reverse mutation assay
Two preincubation assays were conducted.

Evaluation criteria:

An individual trial was judged (+) mutagenic if a dose related increase over the corresponding solvent control was seen, and it was judged weakly mutagenic (+W) if a low dose response was seen. A trial was considered questionable (?) if a dose related increase was judged insufficiently high to justify a call of "+W" if only a single dose was elevated over control or if a non-dose related increase was seen. A chemical was judged weakly mutagenic "+W' or mutagenic "+" if it produced a reproducible, dose related increase in his+ revertants over the corresponding solvent controls in replicate trials.

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Additional information on results:

Testing laboratory: CWR
S9 mix from Aroclor 1254 induced Sprague Dawley rat liver and male Syrian Hamster liver (10% in test concentration)

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results: negative

Using a valid scientific method, n-Decyl methacrylate was negative for genotoxicity in the Salmonella typhimurium reverse mutation assay.

Executive summary:

Using a valid scientific method, n-Decyl methacrylate was negative for genotoxicity in the Salmonella typhimurium reverse mutation assay.