1,3,5-Triazin-2-amine, 4-methoxy-6-(trifluoromethyl)-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

2001

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

2001

Reference 2

Reference Type:

other: Draft Renewal Assessment Report under Regulation (EC) 1107/2009

Title:

Draft Renewal Assessment Report under Regulation (EC) 1107/2009 - Tritosulfuron

Year:

2018

Bibliographic source:

Draft Renewal Assessment Report under Regulation (EC) 1107/2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: EEC 79/831

Principles of method if other than guideline:

No deviations from the current guideline.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

4-methoxy-6-(trifluoromethyl)-1,3,5-triazin-2-amine

EC Number:

610-962-9

Cas Number:

5311-05-7

Molecular formula:

C5H5F3N4O

IUPAC Name:

4-methoxy-6-(trifluoromethyl)-1,3,5-triazin-2-amine

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Details on test solutions:

5 test concentrations plus control, 4 replicates per concentration with 5 daphnid in each; assessment of immobility after 24 hours and at the end of the test after 48 hours. Control, 10, 18, 32, 56 and 100 mg/L (nominal). Glass vessels; test volume 50 mL,

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Waterflea (Daphnia magna STRAUS), neonates collected from in house culture, age at test initiation less than 24 hours; culture conditions in accordance with test conditions.

Study design

Test type:

static

Water media type:

other: synthetic water “M4” (Elendt medium)

Total exposure duration:

48 h

Test conditions

Hardness:

total hardness 2.52 mmol/L

Test temperature:

20 °C – 21 °C

pH:

8.1 - 8.2

Dissolved oxygen:

8.4 - 8.5 mg/L

Conductivity:

655 μS/cm

Details on test conditions:

photoperiod 16 hours light : 8 hours dark; light intensity: < 1500 lux; no feeding, no ventilation

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Any other information on results incl. tables

Tables are attached separately

Applicant's summary and conclusion

Conclusions:

Conclusion of the DAR (2001-2007): 48 h EC50 of > 100 mg a.s./L

Executive summary:

Analytical measurements: Analytical verification of test substance concentration was conducted in each concentration at the beginning and at the end of the test. Measured values at test initiation ranged from 98.9% to 100.8% (average 99.9%) of nominal and from 97.8% to 99% (average 98.6%) of nominal at test termination, confirming the theoretical concentrations. Therefore, the following biological results are based on nominal concentrations.

 

In a 48 hours static acute toxicity study with Daphnia magna the EC50 (48 h) of BH 635-5 was determined to be > 100 mg/L, the EC0 (48 h) was 100 mg/L (nominal).
Biological results: No significant immobility of the daphnids was observed (one immobile daphnia at 32 mg/L after 48 hours). No other substance-related effects were observed.

 

The study was effectively also conducted in compliance with the later version of the guideline, OECD TG 202 (2004), and fulfilled the validty criteria. Analytical measurements to confirm that the test concentrations remained at least 80% of nominal throughout the test period were done. We agree with the endpoint relevant for risk assessment, 48 h EC50 > 100 mg AMTT/L (nom), 48 h NOEC 100 mg AMTT/L (nom).