Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 500-002-6 | CAS number: 9002-92-0 1 - 2.5 moles ethoxylated
Endpoint summary
Administrative data
Description of key information
Hydrolysis
In accordance with column 2 of Annex VIII of the REACH regulation, testing for this endpoint is scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Biodegradation in water
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical (from authoritative database, 2018 and secondary source, 2014). The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l, respectively. The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.
Biodegradation in water and sediment
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end pointis scientifically not necessary and does not need to be conducted since the test chemical is readily biodegradable in water .
Biodegradation in soil
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point is scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Bioaccumulation: aqautic / sediment
In accordance with column 2 of Annex IX of the REACH regulation, testing for this endpoint is scientifically not necessary and does not need to be conducted since the test chemical has a low potential for bioaccumulation based on logKow ≤ 3.
Adsorption / desorption
The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals (Experimental study report, 2019). The solutions of the test substance and reference substances were prepared in appropriate solvents. A test chemical solution was prepared by accurately pipetting 4 microlilter of test chemical and diluted with Methanol up to 10 ml. Thus, the test solution concentration was 367.2 mg/l. The pH of test substance was 7.6. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances were Acetanilide, 4-chloroaniline, 4-methylaniline(p-Tolouidine), N-methylaniline, p-toluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2 -nitrobenzamide, 3 -nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, Benzoic acid methylester, Carbendazim, Benzoic acid phenylester, Xylene, Ethylbenzene, Toluene, Naphthalene, Phenol, N,Ndimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, 1,2,3-trichlorobenzene having Koc value ranging from 1.25 to 3.16. The Log Koc value of test chemical was determined to be 1.643 ± 0.014 dimensionless at 25°C.This log Koc value indicates that the substance has a low sorption to soil and sediment and therefore have moderate migration potential to ground water.
Additional information
Hydrolysis
In accordance with column 2 of Annex VIII of the REACH regulation, testing for this endpoint is scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Biodegradation in water
Various experimental studies of the test chemical were reviewed for the biodegradation end point which are summarized as below:
In an experimental study fromauthoritative database (2018) and secondary source (2014), biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l, respectively. The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.
Another biodegradation study was conducted for 30 days for evaluating the percentage biodegradability of test chemical (HSDB, 2017). The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Initial test substance conc. used in the study was in the range 2-5 mg/l, respectively. The percentage degradation of test chemical was determined to be 74-84% by BOD parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in water.
Estimation Programs Interface Suite (2018) was run to predict the biodegradation potential of the test chemical in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that test chemical is expected to be readily biodegradable.
On the basis of above results of test chemical, it can be concluded that the test chemical is considered to be readily biodegradable in water.
Biodegradation in water and sediment
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end pointis scientifically not necessary and does not need to be conducted since the test chemical is readily biodegradable in water .
Biodegradation in soil
In accordance with column 2 of Annex IX of the REACH regulation, testing for this end point is scientifically not necessary and does not need to be conducted since the test chemicalis readily biodegradable in water.
Bioaccumulation: aqautic / sediment
In accordance with column 2 of Annex IX of the REACH regulation, testing for this endpoint is scientifically not necessary and does not need to be conducted since the test chemical has a low potential for bioaccumulation based on logKow ≤ 3.
Adsorption / desorption
The adsorption coefficient Koc in soil and in sewage sludge of test chemical was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals (Experimental study report, 2019). The solutions of the test substance and reference substances were prepared in appropriate solvents. A test chemical solution was prepared by accurately pipetting 4 microlilter of test chemical and diluted with Methanol up to 10 ml. Thus, the test solution concentration was 367.2 mg/l. The pH of test substance was 7.6. Each of the reference substance and test substance were analysed by HPLC at 210 nm. After equilibration of the HPLC system, Urea was injected first, the reference substances were injected in duplicate, followed by the test chemical solution in duplicate. Reference substances were injected again after test sample, no change in retention time of reference substances was observed. Retention time tR were measured, averaged and the decimal logarithms of the capacity factors k were calculated. The graph was plotted between log Koc versus log k. The linear regression parameter of the relationship log Koc vs log k were also calculated from the data obtained with calibration samples and therewith, log Koc of the test substance was determined from its measured capacity factor. The reference substances were chosen according to estimated Koc range of the test substance and generalized calibration graph was prepared. The reference substances were Acetanilide, 4-chloroaniline, 4-methylaniline(p-Tolouidine), N-methylaniline, p-toluamide, Aniline, 2,5-Dichloroaniline, 4-nitrophenol, 2 - nitrophenol, 2 -nitrobenzamide, 3 -nitrobenzamide, Nitrobenzene, 4-Nitrobenzamide, Benzoic acid methylester, Carbendazim, Benzoic acid phenylester, Xylene, Ethylbenzene, Toluene, Naphthalene, Phenol, N,Ndimethylbenzamide, 3,5-dinitrobenzamide, N-methylbenzamide, Benzamide, 1,2,3-trichlorobenzene having Koc value ranging from 1.25 to 3.16. The Log Koc value of test chemical was determined to be 1.643 ± 0.014 dimensionless at 25°C.This log Koc value indicates that the substance has a low sorption to soil and sediment and therefore have moderate migration potential to ground water.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
