Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-307-5 | CAS number: 24544-08-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April - June 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-compliant guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 2,6-diethyl-p-toluidine
- EC Number:
- 246-307-5
- EC Name:
- 2,6-diethyl-p-toluidine
- Cas Number:
- 24544-08-9
- Molecular formula:
- C11H17N
- IUPAC Name:
- 2,6-diethyl-4-methylaniline
- Test material form:
- other: liquid
- Details on test material:
- - Name of test material (as cited in study report): p-Ditolin
- Physical state: liquid
- Expiration date of the lot/batch: 07-October-1989
- Stability under test conditions: stable
- Storage condition of test material: room temperature, at a dry place
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen (Germany)
- Strain: Bor: WISW (SPF Cpb)
- Age at study initiation: Males ca. 10 weeks and females ca. 15 weeks
- Weight at study initiation: mean males 253 g and females 215 g
- Fasting period before study: 16 hours before treatment
- Housing: gang housing à 5 animals per cage and sex
- Diet (e.g. ad libitum): Altromin® 1324 Pellets (manufacturer: Altromin GmbH und Co KG, Lage, Germany)
- Water (e.g. ad libitum): tap water
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2°C
- Humidity (%): 50 +/- 10%
- Air changes (per hr): 10 changes per hour
- Photoperiod (hrs dark / hrs light): 12 hours (artificial light)
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- peanut oil
- Duration of exposure:
- 24 hours
- Doses:
- 100, 1000 and 2000 mg/kg
- No. of animals per sex per dose:
- 5 animals per sex and dose
- Control animals:
- no
Results and discussion
- Preliminary study:
- Due to high mortality observed in an acute oral toxicity study on rats, 100 and 1000 mg/kg were tested in addition to the limit dose of 2000 mg/kg.
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 703 mg/kg bw
- Based on:
- test mat.
- Mortality:
- 100 mg/kg:
No mortality was observed in both sexes.
1000 mg/kg:
No mortality was noted in males, whereas one female was found dead four days after treatment.
2000 mg/kg:
One out of five male animals was found dead on day 4 after dosing. Three out of five females after 3-4 days after dosing. - Clinical signs:
- other: 100 mg/kg: No treatment-related effects observed. 1000 mg/kg and 2000 mg/kg: Most of males and females at these dose levels showed gasping, dyspnea, chromodacryorrhoe, unsteady gait, bloody snouts and overall bad condition. Clinical signs appeared four
- Gross pathology:
- Animals found dead showed red discolored lungs, bllody snouts and small intestines partly discolored black.
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The acute dermal LD50 on female Wistar rats was found to be 1703 mg/kg.
- Executive summary:
The acute dermal LD50 study was conducted as GLP-study according to OECD no. 402. The test was performed as full test on male and female Wistar rats with doses of 100, 1000 and 2000 mg/kg. Mortality occured at concentrations of 1000 mg/kg and above, slight to moderate clinical symptoms were noted in mid and high dose groups, body weight gain was slightly reduced in all dose groups. At necrospy, a number of treatment-related effects were noted, such as tremor, dyspmea, bloody snouts etc. were recorded in all dose groups.
Therefore, based on the dermal LD50 of 1703 mg/kg in female rats, the test item has to be classified as harmful..
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.