29H,31H-phthalocyanine

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Test guideline 1

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: US FEDERAL REGISTER 38 NO. 187, Paragraph 1500.42 page 27019 of 27. SEPT. 1973

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Brominated chlorinated copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, wherein the number of bromines is equal to or more than 10 and less than 16, and the number of chlorines is equal to or more than 0 and less than 7

EC Number:

701-428-7

Molecular formula:

C32HxBryClzCuN8

IUPAC Name:

Brominated chlorinated copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, wherein the number of bromines is equal to or more than 10 and less than 16, and the number of chlorines is equal to or more than 0 and less than 7

Test material form:

solid: particulate/powder

Details on test material:

green powder

Specific details on test material used for the study:

Heliogengrün 9140

Test animals / tissue source

Species:

rabbit

Strain:

other: Albino

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: 3 - 4 kg
- Housing: single
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 3 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23°C
- Humidity (%): 45 - 65%
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

100 mg

Duration of treatment / exposure:

single application

Observation period (in vivo):

7 days

Number of animals or in vitro replicates:

6

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing (if done): no washing after application


SCORING SYSTEM: Draize
Time points investigated: 1h, 24h, 72h, and longer if needed

TOOL USED TO ASSESS SCORE: not specified

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

cornea opacity score

Basis:

other: all six animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

other: Not applicable, since no findings

Results 2

Irritation parameter:

iris score

Basis:

other: all six animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

other: Not applicable since no adverse findings

Results 3

Irritation parameter:

conjunctivae score

Basis:

other: all six animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

other: Not applicable, since no adverse findings

Results 4

Irritation parameter:

chemosis score

Basis:

other: all six animals

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

other: Not applicable since no adverse findings

Irritant / corrosive response data:

One hour after the application, two rabbits showed slight wetness of the treated eyes.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met