29H,31H-phthalocyanine

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979-11-01 to1980-08-21

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Data from reliable source, non GLP, testing above water solubility

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Principles of method if other than guideline:

"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

GLP compliance:

no

Specific details on test material used for the study:

Purity: >= 99 %

Radiolabelling:

not specified

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Chemical name of vehicle: Crystal sugar, HCO-40
- Concentration of vehicle: 1st Concentration area: Crystal sugar 250 ppb(w/v), HCO-40 250 ppb(w/v), 2nd Concentration area: Crystal sugar 25 ppb(w/v), HCO-40 25 ppb(w/v)

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g
- Lipid content of fish: 2 - 6 %

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test temperature:

25 ± 2 °C

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volum = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 ml/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week

Nominal and measured concentrations:

0.01 mg/l and 0.1 mg/l

Key result

Type:

BCF

Value:

>= 2.1 - <= 74 L/kg

Remarks on result:

other: Conc.in environment / dose:0.01 mg/l

Key result

Type:

BCF

Value:

>= 0.51 - <= 4.8 L/kg

Remarks on result:

other: Conc.in environment / dose:0.1 mg/l

Table 1. Test results

1^stConcentration area	Test substance concentration (ppb (w/v)))	98.4	96.1	97.1	96.7
	Bioconcentration factor (L/kg)	3.1 / 1.3	1.6 / 0.92	0.51 / 1.7	4.8 / 1.8
2^ndConcentration area	Test substance concentration (ppb (w/v)))	8.95	8.41	8.57	8.67
	Bioconcentration factor (L/kg)	4.8 / 74	2.1 / 4.4	2.1 / 2.1	4.6 / 2.1

 

Reference 2

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1979-08-27 to 1979-12-05

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Data from reliable source. Though no detailed test report is available, all tests from the Japanese Authorities are performed according to an OECD guideline and GLP incl. analytics.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Principles of method if other than guideline:

"Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

GLP compliance:

no

Specific details on test material used for the study:

Purity: >= 94 %

Radiolabelling:

not specified

Vehicle:

yes

Details on preparation of test solutions, spiked fish food or sediment:

Chemical name of vehicle: Crystal sugar, HCO-40
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances) - Chemical name of vehicle: HCO-40, Crystal sugar, - Concentration of vehicle: 　1st Concentration area: HCO-40 15 ppm(w/v), Crystal sugar 15 ppm(w/v), 　2nd Concentration area: HCO-40 1.5 ppm(w/v), Crystal sugar 1.5 ppm(w/v)
Test organisms

Test organisms (species):

Cyprinus carpio

Details on test organisms:

TEST ORGANISM
- Common name: Common carp
- Source: obtained from a commercial fish farm in Japan
- Length at study initiation (lenght definition, mean, range and SD): 10 cm
- Weight at study initiation (mean and range, SD): 30 g
- Lipid content of fish: 2 - 6 %

ACCLIMATION
- Acclimation conditions (same as test or not): flow through at 25 ± 2 °C
- Type and amount of food: obtained from a commercial fish farm in Japan at a rate of 2% of the total body weight
- Feeding frequency: daily, no feeding at the day of sampling

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

6 wk

Test temperature:

25 ± 2 °C

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass tanks, volum = 100 L
- Renewal rate of test solution (frequency/flow rate): 200 - 800 ml/min
- No. of vessels per concentration (replicates): 15 - 20
- No. of vessels per control / vehicle control (replicates): 15 - 20


TEST MEDIUM / WATER PARAMETERS
- Holding medium different from test medium: no
- Intervals of water quality measurement: twice a week

Nominal and measured concentrations:

0.01 mg/l and 0.1 mg/l

Key result

Type:

BCF

Value:

<= 3.6 L/kg

Remarks on result:

other: Conc.in environment / dose:0.06 mg/l

Key result

Type:

BCF

Value:

>= 0.33 - <= 11 L/kg

Remarks on result:

other: Conc.in environment / dose:0.6 mg/l

Table 1. Test results

1^stConcentration area	Test substance concentration (ppb (w/v)))	98.4	96.1	97.1	96.7
	Bioconcentration factor (L/kg)	3.1 / 1.3	1.6 / 0.92	0.51 / 1.7	4.8 / 1.8
2^ndConcentration area	Test substance concentration (ppb (w/v)))	8.95	8.41	8.57	8.67
	Bioconcentration factor (L/kg)	4.8 / 74	2.1 / 4.4	2.1 / 2.1	4.6 / 2.1

 

Description of key information

The substance has low bioaccumulative potential with a BCF ≤ 74 L/kg.

Additional information

All substances in the category are inert, insoluble in water and octanol, and unreactive. They do not interact with proteins, have a high molecular weight and are insoluble. Thus, they will not be able to penetrate biological membranes. Therefore, the bioaccumulative potential of these substances is expected to be low. The available experimental data can be used for read-across to all substances in this category. The low bioaccumulative potential conclusion is supported by the available studies for the following two substances in the category: CAS 147-14-8 and CAS 1328-53-6.

Both studies were performed according to OECD TG 305C with Cyprinus carpio as the test species. Test organisms were exposed to different concentrations of the substances for 6 weeks in a flow-through system. For the substance CAS 147-14-8, the BCF was determined to be ≤ 3.6 L/kg for a concentration of 0.06 mg/L treatment. Whereas a concentration of 0.6 mg/L treatment resulted in BCFs in a range between 0.33 and 11 L/kg (NITE 1979, K2). Similar results were obtained for substance CAS 1328-53-6. The test organisms were exposed to 0.1 mg/L and 0.01 mg/L of the test item. The BCFs were determined to be in a range between 0.51 and 74 L/kg (NITE 1992, K2). 

Based on the findings, all substances in this category are expected to have low bioaccumulative potential with a BCF ≤ 74 L/kg.