Registration Dossier
Registration Dossier
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A mixture of: 4-(2,2,3-trimethylcyclopent-3-en-1-yl)-1-methyl-2-oxabicyclo[2.2.2]octane; 1-(2,2,3-trimethylcyclopent-3-en-1-yl)-5-methyl-6-oxabicyclo[3.2.1]octane; spiro[cyclohex-3-en-1-yl-[(4,5,6,6a-tetrahydro-3,6',6',6'a-tetramethyl)-1,3'(3'aH)-[2H]cyclopenta[b]furan]; spiro[cyclohex-3-en-1-yl-[4,5,6,6a-tetrahydro-4,6',6',6'a-tetramethyl)-1,3'(3'aH)-[2H]cyclopenta[b]]furan]
EC number: 422-040-1 | CAS number: 426218-78-2 CASSIFFIX
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
The biodegradability of the substance is tested in a Closed Bottle Test according to OECD TG 301D and in compliance with GLP (Huntingdon, 1995). Sealed bottles containing the test substance (adsorbed onto glass filter paper) at a nominal concentration of 5.6 mg/L were inoculated with activated sewage sludge bacteria and inorganic nutrient medium for up to 28 days. On Days 0, 5, 15 and 28 duplicate bottles were taken and dissolved oxygen measurements were performed electrochemically. Percentage biodegradation values were determined by comparing the extent of oxygen depletion with the Theoretical Oxygen Demand (6.02 mg O2/L). The reference substance sodium benzoate attained 90% biodegradation after 15 days thereby confirming the suitability of the inoculum and test conditions. In a toxicity control containing the test substance and the reference substance, the substance was found to be not inhibitory to activated sewage sludge bacteria. The validity criteria of the test were met. The test substance attained only 3% biodegradation after 28 days. Therefore, the substance is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
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