Reaction mass of Cuprate(4-), [4,5-dihydro-4-[[8-hydroxy-7-[[2-hydroxy-5-methoxy-4-[[2-(sulfooxy)Vinyl]phenyl]azo]-6-sulfo-2-naphthalenyl]azo]-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylato(6-)], trisodium salt and Cuprate(4-), [4,5-dihydro-4-[[8-hydroxy-7-[[2-hydroxy-5-methoxy-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]azo]-6-sulfo-2-naphthalenyl]azo]-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylato(6-)]-, sodium and Cuprate(4-), [4,5-dihydro-4-[[8-hydroxy-7-[[2-hydroxy-5-methoxy-4-[2-(sulfooxy)Ethanol]phenyl]azo]-6-sulfo-2-naphthalenyl]azo]-5-oxo-1-(4-sulfophenyl)-1H-pyrazole-3-carboxylato(6-)], trisodium salt

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates: EC0 (48h) = 1 mg/l, EC50 (48h) = 10 - 100 mg/l

Toxicity to aquatic plants other than algae: EC50 (7d) > 1000 mg/l for growth rate of both frond number and biomass

Additional information

The assessment on aquatic toxicity of target substance was done using data on the substance itself as well as data on 2 read across substances.

In particular, target substance was tested for toxicity to aquatic plants, while 2 read across substances were tested for short-term toxicity to aquatic invertebrates.

In particular, RA Substance 01 was used as read across to assess toxicity to aquatic invertebrates; data on RA Substance 02 is used to account for possible effects caused by a small portion of target substance not present in RA Substance 01.

A detailed description of the read across approach is attached in section 13.

Target substance is very soluble in water, i.e. > 100 mg/l at 20 °C. Similarly, read-across substances are highly soluble in water and non readily biodegradable.

Aquatic invertebrates

A 48 -hour static study with RA Substance 01 was carried out to assess acute toxicity to daphnia magna, as a limit test. Toxic effects in terms of daphnia immobilisation were seen and EC50 (48h) was found to be between 10 and 100 mg/l.

In a 48 -hour study on RA Substance 02, an EC50 > 125 mg/l was found.

Aquatic plants

Toxicity to aquatic algae was assessed in a 7 -day static study, based on OECD guideline 221. Target substance was tested at concentrations of 1000, 316, 100, 31.6 and 10 mg/l of a.i..

EC50 values for growth rate of frond number and biomass were above 1000 mg/l.

Justification for classification or non-classification

According to the CLP Regulation (EC 1272/2008), a substance shall be classified as category acute 1 (H400) when:

96 hr LC50 (for fish) < 1 mg/l and/or

48 hr EC50 (for crustacea) < 1 mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants) < 1 mg/l.

 

Moreover, if adequate chronic toxicity data is not available, a substance shall be classified as category chronic 3 (H412) when:

96 hr LC50 (for fish): 10 - 100 mg/l and/or

48 hr EC50 (for crustacea): 10 - 100 mg/l and/or

72 or 96 hr ErC (for algae or other aquatic plants): 10 - 100 mg/l

and the substance is not rapidly degradable and/or the experimentally determined BCF ≥ 500 (or, if absent, the log Kow ≥ 4).

The decision on classification was derived from experimental data on target substance as well as on read across substances. Based on available data, classification in category chronic 3 (H412) was applied.