3-iodo-2-propynyl butylcarbamate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-03-14

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: P-2848-8603-P100

Analytical monitoring:

yes

Details on sampling:

Analysis of the test item for stability over 48 h was conducted in advance of the study. 100 mL of each dilution was allocated for analysis of the test item at the beginning and the end of the study.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: weighed aliquots of the test item were added to Daphnia Synthetic Media and made up to the required volume in glass volumetric flasks. The test item in Daphnia Synthetic Media was dispersed in a sonic water bath for one hour.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Daphnia were bred within the laboratory by acyclical parthenogenesis and the individuals used were between 6 and 24 h old
- Feeding during test: no

ACCLIMATION
- Acclimation conditions: same as test
- Type and amount of food: culture of Chlorella vulgaris and active yeast
- Health during acclimation: no mortality observed

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

approx. 169 mg/L

Test temperature:

20 +/- 2 °C

Nominal and measured concentrations:

5.0, 2.5, 1.25, 0.625, 0.3125 ppm

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Daphnia Synthetic Medium

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark

EFFECT PARAMETERS MEASURED:
Recording of dead daphnids at 0, 3 , 6, 24, and 48 h of exposure

RANGE-FINDING STUDY
- Test concentrations: 1000, 100, 10, 1, 0.1 ppm

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

0.645 other: ppm

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: equivalent to 0.645 mg/L

Details on results:

- Mortality of control: no
- Other adverse effects control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no

Results with reference substance (positive control):

not applicable

Reported statistics and error estimates:

LC50 values at 24 and 48 h woth 95 % confidence intervals, were calculated by probit analysis.

Table 1 Determination of acute toxicity to daphnids (48 h static)

Measured Concentration of test item (ppm)	Number of Mortalities at Time (h) of exposure
	0	3	6	24	48
5.19	0	0	1	19	20
1.94	0	0	0	18	20
0.740	0	0	0	0	17
0.515	0	0	1	1	1
0.448	0	0	0	0	0
0	0	0	0	0	0

 

Validity criteria fulfilled:

not specified

Conclusions:

The median lethal measured concentration (LC50) of the test item to Daphnia magna has been determined after a 48 h period to be:
48 h LC50 = 0.645 ppm; lower limit: 0.596 ppm; upper limit: 0.696 ppm
Ninety five percent confidence intervals were determined by probit analysis.

Executive summary:

This study was designed to determine the median lethal concentration (LC50) of the test item to Daphnia magna.The study was conducted over a 48 h period under static conditions. Concentrations of 5.0, 2.5, 1.25, 0.625, 0.3125 ppm were applied to four replicates with five daphnids per vessel, respectively. Observations of mortality was performed at 0, 3, 6, 24, and 48 h of exposure. LC50 value with 95 % confidence intervals were calculated with probit analysis. At the two highest concentrations all daphnids died within 48 h of exposure, whereas at the lowest concentration and non-treated control all daphnids survived. LC50 was calulated to be 0.645 ppm.

Description of key information

In an 48 h acute toxicity study with daphnids under static conditions an LC50 value of 0.645 ppm was determined.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.645 mg/L

Additional information

Acute toxicity to aquatic invertebrates

This study was designed to determine the median lethal concentration (LC50) of the test item to Daphnia magna.The study was conducted over a 48 h period under static conditions. Concentrations of 5.0, 2.5, 1.25, 0.625, 0.3125 ppm were applied to four replicates with five daphnids per vessel, respectively. Observations of mortality was performed at 0, 3, 6, 24, and 48 h of exposure. LC50 value with 95 % confidence intervals were calculated with probit analysis. At the two highest concentrations all daphnids died within 48 h of exposure, whereas at the lowest concentration and non-treated control all daphnids survived. LC50 was calulated to be 0.645 ppm.