29H,31H-phthalocyanine

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Name of test material (as cited in study report): Heliogen Gruen 8730 Pulver;
- Source and lot/batch number of test material: BW137(Partien 616-619)
- Analytical purity: > 98%, Impurities: 0.11% water


STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: ambient

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

The test substance was weighed and added directly to each parallel. No dilution series was prepared

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: Eisenia fetida (Michaelsen)
- Source:
- Age at test initiation (mean and range, SD): 9 months
- Weight at test initiation (mean and range, SD): 300 - 600 mg

ACCLIMATION
- Acclimation period: 24 h before the test, in test substrate without test substance
- Acclimation conditions (same as test or not): same

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test temperature:

19.3 - 20.3 °C

pH:

6.3

Moisture:

- water content(day 0): 1563.8 g (33.3 %) - water content(day 14): 1561.6 g (33.2 %)

Details on test conditions:

TEST SYSTEM
- Test container (material, size): 1l glass jars with glass lids
- Amount of soil or substrate: 750 g artificial soil (dry weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No.r of parallels without animals: 1 (determination of the water content, pH and temperature measurement)


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil)
- % sand: 69.7
- % sphagnum peat: 10
- % clay: 20


OTHER TEST CONDITIONS
- Light intensity: continuous illumination, about 400 - 800 lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality after 7 and 14 days, (inhibition of the biomass)

VEHICLE CONTROL PERFORMED: no

Nominal and measured concentrations:

Nominal: 0, 62.5, 125, 250, 500, 1000 mg/kg

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The short hatching of the earthworms before introduction in the test was carried out with a pH-value of 4.1. However this has no influence on the EC-value of the reference substance, the weight and the clitellum of the earthworms.

Validity criteria:

- The mortality in the controls did not exceed 10 % at the end of the test.

- The LG50 <14d) of the control substance chloracetamide was 25,9 mg/kg.

Validity criteria fulfilled:

yes

Description of key information

The substance is not toxic to soil microorganisms except arthropods, with an LC50 > 1000 mg/kg soil dey weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw

Additional information

All substances in this category share a similar structure with a molecular weight > 500 g/mol. Based on the high molecular weight and insolubility, the substances in this category will not be able to penetrate biological membranes. Thus, no systemic exposure is expected. This is confirmed by the environmental studies on soil microorganisms except arthropods for one substance in this category: CAS 1328-53-6. The environmental toxicity data can be used for read-across to all substances in this category.

In a guideline study according to OECD TG 207 on Eisenia foetida, the substance CAS 1328-53-6 was mixed with artificial soil, and the earthworms were kept on the soil/substance mixture for 14 days. The 14-d NOEC was determined to be ≥ 1000 mg/kg soil (BASF 2000, K2). The results indicate that the substance is not toxic to soil macroorganisms (arthropods excluded).

In addition to the experimental data, all substances in this category have a low potential for adsorption to soil and no bioaccumulative properties. Therefore, binding to sewage sludge is unlikely. Consequently, a transfer to the soil compartment is not expected. The lack of harmful effects to all three trophic levels of aquatic organisms at concentrations below the solubility in water indicates a low hazardous potential of the test substance towards soil organisms. Based on the available information, it is concluded that all substances in this category are not toxic to soil microorganisms except arthropods, with an LC50 > 1000 mg/kg soil dry weight.