methyl (2E)-[2-(chloromethyl)phenyl](methoxyimino)acetate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 520 F - Chlormethyloximether
- Physical state: solid/ dark green
- Analytical purity: 94.4 g/100 g
- Batch No.: 31196-182
- Storage condition of test material: ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance is not stable and dissolvation in water is slow. Stock dispersions were prepared for each 24 hour renewal. The stock dispersions were treated by ultra sonification for 30 minutes. No prolonged stirring interval was used to avoid complete hydrolysis before the start of exposure.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: 2 - 24 h (starting with the 3rd breed of parent animals)
- Feeding during test: none

BREEDING CINDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Temperature: 20 ± 2 °C
- Diet: Desmodesmus subspicatus
- Feeding: Daily
- Renewal of the breeding water: Monday, Friday

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.41 mmol/L

Test temperature:

20.3 - 20.7 °C

pH:

7.7 - 8.8

Dissolved oxygen:

7.1 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 6.25, 12.5, 25, 50, 100 mg/L
Mean measured test concentrations: -, 86.1, 38.9, 20.0, 9.85, 4.85 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass tubes with flat bottom, 20 ml, 10 ml
- Renewal rate of test solution: daily
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.5 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water (M4 medium)
- Alkalinity: 0.86 mmol/L
- Conductivity: 608 µS/cm
- Intervals of water quality measurement:
- Temperature: Continuously during the whole test period in a separate vessel close to the test vessels.
- pH and oxygen: After 0h and 24h (new test solutions) in an additional parallel without daphnids and after 24 h and 48h (old test solutions) in parallel 1 of each tested concentration

OTHER TEST CONDITIONS
- Photoperiod: day:night rhythm = 16 : 8 h
- Light intensity: Artificial light, type warm white; about 1 - 8 μE/m²s at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED: Swimming ability of the test animals after 0, 24 and 48 days

TEST CONCENTRATIONS
- Range finding study: yes
- Results used to determine the conditions for the definitive study: EC50 range derived from 48 hour range finding: 10 - 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

6.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

21.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 5.92 - 101 mg/L

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The 48 hour EC50 value for in the Daphnia magna study were 16.7 mg/L based on the mean of analytically determined concentrations.

Any other information on results incl. tables

For an evaluation of the results the nominal concentrations should be used, since the effects observed in this test include effects of undissolved test substance and of the products of hydrolysis.