methyl (2E)-[2-(chloromethyl)phenyl](methoxyimino)acetate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): 520 F - Chlormethyloximeter
- Physical state: solid/ dark green
- Analytical purity: 94.4 g / 100 g
- Batch No.: 31196-182
- Storage condition of test material: ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance is not stable in water and the dissolvation is slow. The test concentrations were prepared without a prolonged stirring period to avoid hydrolysis before start of exposure. Undissolved test substance was not removed, so that the dissolvation process could proceed. The test results can be attributed to the test substance and to the hydrolysis products that were not analysed. For preparing the test solutions the test substance was stirred for approx. 1 hour in the test water. Each test concentration was prepared separately.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Source: Hatch at the laboratory for Experimental Toxicology and Ecology
- Age at study initiation: 14 months
- Length at study initiation (length definition, mean, range): from the top of the snout to the end of the caudal fin, 3.6 cm (3.0 – 4.3 cm)
- Weight at study initiation (wet weight): 0.45 g (0.22 – 0.94 g)
- Feeding during test: none

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Photoperiod: 16 hours light, 8 hours dark
- Type and amount of food: Commercial fish diet „Tetramin“, supplier Tetra-Werke, Melle, Germany, ad libitum, additionally generally on workdays live brine shrimp (artemia).
- Health during acclimation: no signs for sickness, injuries or abnormalities were observed
- Mortality during the last week before start of exposure: 0 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

100 mg/L CaCO3

Test temperature:

23 °C

pH:

8.0 - 8.4

Dissolved oxygen:

8.1 - 8.8 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 10, 22, 50, 100, 150 mg/L
Mean measured concentrations: -, 3.18, 6.18, 13.5, 45.3, 63.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass aquaria, 30 cm long, 22 cm wide, 24 cm high, 10 L
- Aeration: slight
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 7
- No. of vessels per control (replicates): 7
- Biomass loading rate: 0.32

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Non chlorinated charcoal filtered tap water mixed with deionized water, aerated
- Total hardness: Approx. 1 mmol/L = 100 mg/L CaCO3
- Conductivity: Approx. 250 μS/cm (at 25°C)
- Ca content: Approx. 40 mg/L
- Mg content: Approx. 5 mg/L
- Acid capacity: Approx. 2.5 mmol/L
- pH-value: Generally 7.5 – 8.5
- Intervals of water quality measurement: Temperature, oxygen content and pH-value were measured in each test vessel within 1 hour after start of exposure and after 24, 48, 72 and 96 hours

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light, 8 hours darkness
- Light intensity: Approx. 36 - 191 Lux (the light intensity is determined in regular intervals at the surface of the aquaria)

EFFECT PARAMETERS MEASURED: Survival and toxic signs (changes appearance, swimming behaviour and behaviour in comparison to the control group) within 1 hour after start of exposure and after 4, 24, 48, 72, 96 hours

TEST CONCENTRATIONS
- Range finding study: yes (non GLP)
- Test concentrations: LC50 > 100 mg/L
- Results used to determine the conditions for the definitive study: The highest test concentration 150 mg/L was selected because of the instability of the test substance

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

96.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Details on results:

- Mortality of control: none
- Behavioural abnormalities:
- 50 mg/L treatment group: 4 fish were tottering after 1 and 4 hours and 2 fish after 24 hours
- 100 mg/L treatment group: 2 fish were tottering after 1 hour and 7 fish after 4 and 24 hours; 5 fish were swimming at the bottom after 1 hour, 3 fish after 72 hours and 2 fish after 96 hours (the remaining fish were dead)
- 150 ml treatment group: 7 fish were tottering and swimming at the bottom after 1 hour; 2 fish showed apathy after 4 hours
- Dissolution behaviour: homogeneous dispersion of the test substance in water, turbidity; undissolved test substance at the bottom of the test vessel and undissolved test substance on the water surface in the 50, 100 and 150 mg/L treatment groups

Any other information on results incl. tables

Sublethal observations / clinical signs:

The effects observed in this study were the combined effects of the test substance and its products of hydrolysis, which were not analysed. Therefore the evaluation can be based on the nominal concentrations of the test substance.