Registration Dossier
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EC number: 600-337-9 | CAS number: 102691-36-1
Ecotoxicological Summary
Administrative data
Hazard for aquatic organisms
Hazard for air
Hazard for terrestrial organisms
Hazard for predators
Additional information
Conclusion on classification
Two study reports (rated as Klimisch 1) on aquatic organisms are available covering two trophic levels: a primary producer (freshwater algae, represented by the model species Pseudokirchneriella subcapitata) and a primary consumer (aquatic invertebrates, represented by the model species Daphnia magna).
The freshwater algae P. subcapitata was exposed to 2-Cyanoethyl-N,N,N’,N’-tetraisopropylphosphordiamidite for 72 hours in a GLP toxicity study (OECD 201) at 100, 32, 10, 3.2, and 1.0 mg/L. In this study a NOErC of 3.2 mg/L and an ErC50 of 20.4 mg/L were derived. The test concentrations were evaluated analytically by TOC. The relevant measured concentrations were ± 20 % of the nominal values.
D. magna was exposed for 48 hours to 2-Cyanoethyl-N,N,N’,N’-tetraisopropylphosphordiamidite in a semi-static short-term GLP toxicity study (OECD 202) at 0.94, 2.20, 4.31, 9.63, 20.0, 33.2, 62.2 mg test item/L (calculated concentrations based on TOC measurements). In this study a NOEC of 0.96 mg/L and an EC50 of 22.8 mg/L were derived. The test concentrations were evaluated analytically by TOC.
In both studies the concentrations were analytically verified by TOC. The method was chosen because the test item hydrolyses very quickly, and the first hydrolysis product reacts further resulting in a continuous change in hydrolysis products. Therefore, it is not possible to focus on one single hydrolysis product for toxicity testing. In addition, no major and stable hydrolysis product could be found over the course of several days. To comprise all hydrolysis products TOC is the best suitable method for the present study.
Taking into account that 2-Cyanoethyl-N,N,N’,N’-tetraisopropylphosphordiamidite is considered to be not readily biodegradable based on ThCO2 (Hammesfahr 2018) and the lowest EC50 value was determined to be > 10 mg/L and < 100 mg/L (72-h ErC50 = 20.4 mg/L), 2-Cyanoethyl-N,N,N’,N’-tetraisopropylphosphordiamidite has to be classified as "Aquatic Chronic 3" (H412) following the provisions laid down in the CLP Regulation (Regulation (EC) No 1272/2008).
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