Phosphorodithioic acid, O,O-di-dodecyl-esters, zinc salts, neutral and basic

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical measurements of test material. TOC analyticals did not show gradient

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water accommodated fraction (WAFs)
Test material was added to 21 L of water and stirred for 48 hours. The stir rate was set to form a slight dimple at the water surface. After stirring, the mixture was allowed to stand for 4 hours. A wide bore glass tube, covered at one end with Parafilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. The aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates.

- Evidence of undissolved material (e.g. precipitate, surface film, etc): Microscopic inspection of the WAFs showed no micro-dispersions or undissolved test material

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: Brow Well Fisheries Limited, Hebden, Yorkshire, UK
- Length at study initiation (length definition, mean, range and SD): 4.3 cm
- Weight at study initiation (mean and range, SD): 1.07 g
- Food type: Commerical trout pellets. Feeding was discontinued 24 hours prior to start of the study. Food was held for 24 hours.

ACCLIMATION
- Acclimation period: Two weeks
- Acclimation conditions (same as test or not): same as test conditions

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

none

Hardness:

100 mg/l as CaCO3

Test temperature:

14 °C

pH:

7.6 - 8.0

Dissolved oxygen:

9.0 - 9.8 mg Oxygen/L

Salinity:

not applicable/freshwater

Nominal and measured concentrations:

Nominal: 1.0, 1.8, 3.2, 5.6, 10.0 mg/L

Details on test conditions:

TEST SYSTEM
- Type: closed
- Material, size, headspace, fill volume: 20 litre glass vessels
- Aeration: None

- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated laboratory tap water passed through activated carbon filter
- Total organic carbon: 2 mg C/L
- Particulate matter: 0.10 mg/L

- Alkalinity: 104 mg/L as CaCO3

- Conductivity: 473 µS

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light

TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline:
- Range finding study
- Test concentrations: 1.0, 10, 100, and 1000 mg/L
- Results used to determine the conditions for the definitive study:
1.0 mg/L: 0/3
10, 100, and 1000 mg/L: 3/3 for each dose

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 4.4 - 4.6 mg/L

Duration:

96 h

Dose descriptor:

NOELR

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Behavioural abnormalities: Loss of equilibrium in 5.6 mg/L Test group at 48 hours
- Mortality of control: 0/10

Validity criteria fulfilled:

yes

Conclusions:

LL50 (96h) = 4.4 mg/L (OECD 203, Oncorhynchus mykiss, mortality, nominal)

Executive summary:

No data are available on the toxicity of Phosphorodithioic acid, O,O-di-dodecyl-esters, zinc salts, neutral and basic (CAS No.4563-56-8) to fish. Therefore data from the analogue substance Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-8) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5 and justified in detail in the overall summary (IUCLID Chapter 6.1) and within the analogue justification attached in IUCLID Section 13.

The short-term toxicity of Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-9) (Safepharm laboratories Ltd, 2002) to fish was investigated in a study according to OECD 203 and following GLP principles (CAS 4259-15-8, Oronite, Short-term toxicity to fish). In this test, Oncorhynchus mykiss were exposed to nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10.0 mg/L under semi-static conditions. Due to a low solubility of the test item a water accommodated fraction design was performed (stirred for 48 h and allowed to stand for additional 4 h; the aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates). 10 fish per vessel were exposed to the test item loading for a period of 96 h. The LL50 was determined to be at 4.4 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to the analogue justification provided in Section 13.

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 4.4 - 4.6 mg/L

Description of key information

LL50 (96h) = 4.4 mg/L (OECD 203, Oncorhynchus mykiss, mortality, nominal)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4.4 mg/L

Additional information

No data are available on the toxicity of Phosphorodithioic acid, O,O-di-dodecyl-esters, zinc salts, neutral and basic (CAS No.4563-56-8) to fish. Therefore data from the analogue substance zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-8) were used for read across. This read-across is in accordance with Regulation (EC) No. 1907/2006 Annex XI, 1.5 In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment. This read-across is justified in within the analogue justification in IUCLID Section 13.

The short-term toxicity of Zinc bis[O,O-bis(2-ethylhexyl)] bis(dithiophosphate) (CAS 4259-15-9) (Safepharm laboratories Ltd, 2002) to fish was investigated in a study according to OECD 203 and following GLP principles (CAS 4259-15-8, Oronite, Short-term toxicity to fish). In this test, Oncorhynchus mykiss were exposed to nominal loading rates of 1.0, 1.8, 3.2, 5.6 and 10.0 mg/L under semi-static conditions. Due to a low solubility of the test item a water accommodated fraction design was performed (stirred for 48 h and allowed to stand for additional 4 h; the aqueous phase or WAF was removed by mid-depth siphoning to give the necessary loading rates). 10 fish per vessel were exposed to the test item loading for a period of 96 h. The LL50 was determined to be at 4.4 mg/L.