Registration Dossier
Registration Dossier
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EC number: 232-178-2 | CAS number: 7789-60-8
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from HSDB
Data source
Reference
- Reference Type:
- secondary source
- Title:
- Gene mutation toxicity study for Phosphorus tribromide
- Author:
- U. S. National Library of medicine
- Year:
- 2 017
- Bibliographic source:
- Toxicology data network, 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- Gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus tribromide
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phosphorus tribromide
- EC Number:
- 232-178-2
- EC Name:
- Phosphorus tribromide
- Cas Number:
- 7789-60-8
- Molecular formula:
- Br3P
- IUPAC Name:
- phosphorus tribromide
- Test material form:
- liquid
- Details on test material:
- - Name of test material : Phosphorus tribromide
- Molecular formula: Br3P
- Molecular weight : 270.686 g/mol
- Smiles notation :P(Br)(Br)Br
- InChl :1S/Br3P/c14(2)3
- Substance type: Inorganic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: Phosphorus tribromide
- IUPAC name: Phosphorus (3+) tribromide
- Molecular formula: Br3P
- Molecular weight: 270.686 g/mol
- Substance type: Inorganic
- Physical state: Colourless liquid
- Purity: No data
- Impurities (identity and concentrations): No data
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: frame shift or base-pair substitution tester strains
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- not specified
- Metabolic activation system:
- No data
- Test concentrations with justification for top dose:
- No data
- Vehicle / solvent:
- No data
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar
DURATION
- Preincubation period: No data
- Exposure duration: No data
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data
SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data
NUMBER OF REPLICATIONS: No data
NUMBER OF CELLS EVALUATED: No data
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data
OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data
- Other: No data
OTHER: No data - Rationale for test conditions:
- No data
- Evaluation criteria:
- The plates were observed for a dose dependent increase in the number of revertnats/plate
- Statistics:
- No data
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: frame shift or base-pair substitution tester strains
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Remarks:
- in both buffered and unbuffered solutions
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- No data
Applicant's summary and conclusion
- Conclusions:
- Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.
- Executive summary:
Gene mutation toxicity study was performed to determine the mutagenic nature of Phosphorus tribromide. The study was performed using Salmonella typhimurium frame-shift or base-pair substitution tester strains in both buffered and unbuffered solutions. No mutagenic activity was noted in the mentioned strains due to the test chemical.
Phosphorus tribromide did not induce gene mutation in Salmonella typhimurium frame-shift or base-pair substitution tester strains and hence the chemical is not likely to classify as a gene mutant in vitro.
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