Sodium bromide

Short-term toxicity to aquatic invertebrates

Description of key information

Key study: Test method according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The 48h-LC50 values for the different Daphnia magna genotypes (A, A-1, A-2, B, E and S-1) ranged from 6.7 (genotype A-1) to 9.3 g/L (genotypes A, E and S-1).
Key study: Test method equivalent or similar to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). Test performed in accordance with the Dutch Standardization Organization (1980, NEN 6501: Determination of acute toxicity with Daphnia magna). The 48h-LC50 was determined to be 11 g Br-/L (14.1 g NaBr/L) and the NOEC, based on mortality and abnormal behaviour, was 4.3 g Br-/L (5.5 g NaBr/L). 
Supporting study: Study well documented, meets generally accepted scientific principles, acceptable for assessment. The 48h-LC50 was determined to be 8.9 g/L.
Supporting study: No guideline was followed. No analytical monitoring was made. The 24 -h median lethal concentration (LC50) values for sodium bromide with respect to 48 -, and 72 -h-old Artemia were 531.06 mg/L and 42.42 mg/L, respectively. It can be concluded that there is an age-dependent increase in sensitivity for the test compound.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

6.7 g/L

Additional information

Key study: A comparative study was made of the performance of six of the more common genotypes of Daphnia magna in terms of neonate mortality under acute exposure to sodium bromide. The study was performed according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The organisms were exposed for 48 hours to different concentrations of the test substance. They were observed for mortality, recognized by immobility. The LC50 values of the different genotypes included in the study (A, A-1, A-2, B, E and S-1) ranged from 6.7 to 9.3 g/L, being A-1 and B the most sensitive genotypes and A, A-2, E and S-1 the least sensitive ones.

Key study: A 48 hour acute toxicity test in Daphnia magna was performed with sodium bromide, following a method equivalent or similar to the OECD Guideline 202 and in accordance to the Dutch Standardization Organization method (1980: NEN 6501). 25 organisms per vessel were exposed to the test substance and they were observed for mortality and abnormal behaviour. After 48 -hour exposure, the LC50 was determined to be 11 g Br^-/litre (14.1 g NaBr/L), the EC50, based on mortality and abnormal behaviour, was 5.8 g Br^-/litre (7.5 g NaBr/L), the LC0 was stated to be 7.8 g Br^-/litre (10 g NaBr/L) and the NOEC, based on mortality and abnormal behaviour, was determined to be 4.3 g Br^-/litre (5.5 g NaBr/L).

Supporting study: An acute toxicity test in Daphnia magna was performed with sodium bromide. Fifteen to twenty 14 -d-old Daphnia magna organisms per vessel were exposed to six concentrations of sodium bromide plus a control. The effect parameter measured was mortality, which was defined as a lack of response to a bright-light stimulus. After 48 -hour exposure, the LC50 was determined to be 8.9 g/L.

Supporting study: This study reports the acute toxicity of sodium bromide on Artemia larvae. 48 -h-old and 72 -h-old organisms were exposed for 24 hours to the test substance at concentrations ranging from 15 to 1,200 mg/L. Each test concentration was replicated 4 times and involved 10 organisms per vessel. Larvae were considered dead if they showed neither internal nor external movement over a 10 -s observation period. The 24 -h median lethal concentration (LC50) values for sodium bromide with respect to 48 -, and 72 -h-old Artemia were 531.06 mg/L and 42.42 mg/L, respectively. The LC50 obtained showed that the 72 -h-old organisms were significantly less tolerant than the 48 -h-old organisms. Thus, it can be concluded that there is an age-dependent increase in sensitivity for the test compound.