Registration Dossier
Registration Dossier
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EC number: 609-333-1 | CAS number: 37143-54-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-07-04 to 1997-08-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 609-333-1
- EC Number:
- 609-333-1
- Cas Number:
- 37143-54-7
- Molecular formula:
- C4 H11 N O
- IUPAC Name:
- 609-333-1
- Details on test material:
- - Physical state: No data
- Analytical purity: 99.7 %
- Batch number: 080995003
- Manufactured: 1996-10-31
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Mixture of municipal and synthetic sewage taken from laboratory STP
- Concentration of sludge: 30 mg/L dry substance - Duration of test (contact time):
- 35 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 37 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Test temperature: Room temperature
TEST SYSTEM
- Fill volume of test flasks: 1000 mL
- Number of culture flasks/concentration: 2
SAMPLING
- Sampling frequency: On days 0, 1, 3, 5, 7, 9, 13, 16, 20, 23, 27, 31, 34 and 35
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
- Abiotic control: yes
Reference substance
- Reference substance:
- aniline
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (DOC removal)
- Value:
- 40 - 50
- Sampling time:
- 27 d
- Parameter:
- % degradation (DOC removal)
- Value:
- 50 - 60
- Sampling time:
- 35 d
- Details on results:
- - Duration of adaptation phase: 23 d
- Validity criteria met: yes
- 10 day window met: no
- Degradation of inhibition control after 13 days: 40 - 50 %
BOD5 / COD results
- Results with reference substance:
- Degradation of reference substance after 13 days: 90 - 100 % DOC removal
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable according to OECD criteria
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