Benzothiazole-2-thiol

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test within the National Toxicology Program’s mutagenicity testing program and according to GLP.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1987

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

not specified

Type of assay:

bacterial gene mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Benzothiazole-2-thiol

EC Number:

205-736-8

EC Name:

Benzothiazole-2-thiol

Cas Number:

149-30-4

Molecular formula:

C7H5NS2

IUPAC Name:

1,3-benzothiazole-2-thiol

Constituent 2

Reference substance name:

mercaptobenzothiazole

IUPAC Name:

mercaptobenzothiazole

Details on test material:

Vendor purity: 99%
Analyzed purity: 96,3%
Testing lab: Case Western Reserve University CWR; Dr.W.Speck

Method

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Details on mammalian cell type (if applicable):

All strains were obteined from Dr.Bruce ames (Univertity of California, Berkeley).

Metabolic activation:

with and without

Metabolic activation system:

S9 from hamster liver and S9 from rat liver

Test concentrations with justification for top dose:

3.300- 10.000 - 33.000 - 100.000 - 333.000 µg/plate

Controls

Controls 1

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Remarks:

with and without metabolic activation

Positive control substance:

sodium azide

Remarks:

Migrated to IUCLID6: TA 1535 and TA 100

Controls 2

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Remarks:

with and without metabolic activation

Positive control substance:

9-aminoacridine

Remarks:

Migrated to IUCLID6: TA 1537

Controls 3

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Remarks:

with and without metabolic activation

Positive control substance:

other: 4-nitro-o-phenylenediamine. TA 98

Details on test system and experimental conditions:

2 days incubation at 37ºC.
The plates were hand-counted whena precipitate was present; otherwise automatic colony counters were used.

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

The test substance is non mutagenic.

Executive summary:

The aim of the test was to determine the ability of the test material to induce mutations in Salmonella.

The concentrations were: 3.300- 10.000 - 33.000 - 100.000 - 333.000 µg/plate. Mercaptobenzothiazole is non mutagenic.