Registration Dossier
Registration Dossier
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EC number: 203-402-6 | CAS number: 106-48-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- A database of chemical toxicity to environmental bacteria and its use in interspecies comparisons and correlations.
- Author:
- Blum DJW, Speece RE
- Year:
- 1 991
- Bibliographic source:
- Research Journal WPCF 3: 198-207.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - 96h anaerobic toxicity assay with methanogenic bacteria
- 49h toxicity test with activated sludge
- 24h nitrification inhibition test (Nitrosomonas spec.)
- Microtox bioassay (Photobacterium phosphoreum) - GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-chlorophenol
- EC Number:
- 203-402-6
- EC Name:
- 4-chlorophenol
- Cas Number:
- 106-48-9
- Molecular formula:
- C6H5ClO
- IUPAC Name:
- 4-chlorophenol
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- other: methanogens; activated sludge; Nitrosomonas; Photobacterium phosphoreum
Study design
- Test type:
- not specified
- Water media type:
- freshwater
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- IC50
- Effect conc.:
- 270 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- other: Inhibition of gas production
- Remarks on result:
- other: Methanogenic bacteria
- Duration:
- 49 h
- Dose descriptor:
- IC50
- Effect conc.:
- 98 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: Activated sludge
- Duration:
- 24 h
- Dose descriptor:
- IC50
- Effect conc.:
- 0.73 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: Nitrosomonas spec.
- Duration:
- 5 min
- Dose descriptor:
- IC50
- Effect conc.:
- 0.95 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: Inhibition of bioluminescence
- Remarks on result:
- other: Microtox test
Applicant's summary and conclusion
- Executive summary:
Blum and Speece (1991) published several results of (i) a 96h anaerobic toxicity assay with methanogenic bacteria, (ii) a 49h toxicity test with activated sludge, (iii) a 24h nitrification inhibition test (Nitrosomonas spec.) and (iv) of Microtox bioassay (Photobacterium phosphoreum). Obtained results are (i) IC50 270 mg/L, (ii) IC50 98 mg/L, (iii) IC50 0.73 mg/L, (iv) IC50 0.95 mg/L. Regarding these tests, no guideline was followed.
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