4-hydroxybutyl acrylate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Principles of method if other than guideline:

BASF Test: Several groups of 5 NMRI (Ivanova) mice per sex and dose were treated simultaneously i.p. with aqueous solutions of the test substance. Group-wise documentation of clinical signs was performed over the 14-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form.
On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-hydroxybutyl acrylate

EC Number:

219-606-3

EC Name:

4-hydroxybutyl acrylate

Cas Number:

2478-10-6

Molecular formula:

C7H12O3

IUPAC Name:

4-hydroxybutyl acrylate

Specific details on test material used for the study:

Test substance: Butanediolmonoacrylate
Chemical name: 2-Propenoic acid, 4-hydroxy ester

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Ivanova
- Mean body weights at study initiation: males 26.7 g, females 24.0 g

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Details on exposure:

MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg bw

Doses:

1470, 1000, 681, 464, 316, and 215 µL/kg bw (corresponding to approx. 1529, 1040, 708, 483, 329, and 224 mg/kg bw)
Recalculation based on density = 1.04 g/cm3 at 25 °C (BASF AG, MSDS, 2007)

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
Cageside observations were performed several times on the day of administration and daily on workdays during the observation period. Body weights were determined at test start only.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 483 - 708 mg/kg bw

Based on:

test mat.

Mortality:

At the two highest doses all treated animals died within the first hour after substance administration. At 708 mg/kg bw 7/10 animals died within 24 hrs, after 48 hrs 10/10 animals had expired. At the three lower doses, no mortality occurred.

Clinical signs:

Signs of toxicity comprised dyspnoea, apathy, lateral-abdominal position, staggering, twitching, saltatory convulsions, tremor, fibrillary convulsions, clonic convulsions, and poor general state.

Body weight:

Body weight gain during the course of the study was reported to be normal.

Gross pathology:

Deceased animals: liver degeneration.
Sacrificed animals: no adverse abnormalities observed at gross-pathological examination.

Any other information on results incl. tables

Original value: LD50 > 460 < 680 µL/kg bw; therefore LD50 > approx. 478 < approx. 707 mg/kg bw (density = 1.04 g/mL).

Mortality:

Dose [mg/kg bw]	Conc. [%]	No. of animals/sex	Dead animals / treated animals after
			1 h	24 h	48 h	7 d	14 d
1529	14.7	5 m	5/5	5/5	5/5	5/5	5/5
		5 f	5/5	5/5	5/5	5/5	5/5
1040	10	5 m	5/5	5/5	5/5	5/5	5/5
		5 f	5/5	5/5	5/5	5/5	5/5
708	6.81	5 m	0/5	4/5	5/5	5/5	5/5
		5 f	0/5	3/5	5/5	5/5	5/5
483	4.64	5 m	0/5	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5	0/5
329	3.16	5 m	0/5	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5	0/5
224	2.15	5 m	0/5	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5	0/5

f: female

m: male