polymeric zinc propylenebis(dithiocarbamate);propineb (ISO)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998/05/04 to 1998/10/01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

Deviations:

yes

Remarks:

Although the analytical results are partly less than 80 % of nominal the acute toxicity is based on nominal concentrations.

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

HPLC-UV

Details on sampling:

The samples were taken at the end of the test and at test termination. The samples were stored in a refrigerator until they were analysed.

Vehicle:

no

Details on test solutions:

Test concentrations were 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L (nominal).

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

All test fish were held in culture tanks on a 16/8 hour light/dark photoperiod and observed for at least 14 days prior to testing. Less than 3 % mortality was noted prior to the test initiation and all unsuitable fish (e.g. injured, deformed, etc.) were eliminated from inclusion in the test prior to assignment to test groups. A continuous record offish observations during the holding period, along with any prophylactic and therapeutic disease treatments, if applicable, is included in the raw data of the fish lot.

During the acclimation period they were fed a commercial trout diet. They were not fed 48 h before and during the study. The feed was not analyzed for unwanted contaminants. In this test mean body wet weight of the fish at the beginning of the test was 0.7 ± 0.1 g (Mean ±SD), mean body total length was 4.2 + 0.2 cm (Mean + SD). The biomass loading was 0.18 g fish/L test medium.

Test type:

static

Water media type:

other: Reconstituted water prepared by adding salt stock solutions to demineralized water

Total exposure duration:

96 h

Hardness:

40 - 60 mg CaCO3/L

Test temperature:

12.3°C - 12.5°C

pH:

7.0 - 7.3

Dissolved oxygen:

97% - 101%

Nominal and measured concentrations:

Nominal concentrations: 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L, Mean measured concentrations: 0.082, 0.182, 0.368, 0.723, 1.29 and 2.54 mg a.s./L

Details on test conditions:

The aquaria were made of glass and had a size of 32 x 36 x 38 cm (l x d x h). The test volumes
amounted to 40 L. For every test concentration one aquarium was used, labelled with study number, a consecutive number and the nominal concentration of the test substance. The aquaria were placed in a temperature controlled room. Immediately before the start of the test water samples were taken from the center of the aquaria for analytical determination of the active ingredient concentration.

At the start of the test, ten fish were introduced into each aquarium in an order determined by a table of random numbers. The time between preparing the test solution and the addition of fish were 30 min. During the test, fish were examined after four hours and then daily for mortalities and symptoms of intoxication. In case 100% mortality was reached in test concentrations prior to the end of the test, the analytical determinations were made at those times.

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

329 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

0.103 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

0.206 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Study was performed with the formulated product Antracol U with a active substance content of 82.3%.

Validity criteria fulfilled:

not specified

Conclusions:

An acute 96 h toxicity test was conducted to estimate the toxicity of the test substance to Rainbow Trout (Oncorhynchus mykiss). Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L. Based on nominal concentrations of propineb, the LC50 (96 h) was determined to be 0.40 mg a.s./L.

Executive summary:

An acute 96 h toxicity test was conducted to estimate the toxicity of the test substance to Rainbow Trout (Oncorhynchus mykiss). The primary measure for acute toxicity was mortality. Sublethal and behavioural observations were made during the course of the study. Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L.

In test water mean measured values for propineb between 77% and 89% of nominal were found. There were neither symptoms of intoxication nor mortality in the control group. The lowest lethal concentration (LLC) was 0.50 mg a.s./L. The lowest-observed-effect concentration (LOEC) was 0.25 mg a.s./L. The minimum concentration causing 100% mortality (96 h) was 1.00 mg a.s./L. The no-observed-effect-concentration (NOEC) was 0.125 mg a.s./L. Based on nominal concentrations of propineb, the LC₅₀ (96 h) was determined to be 0.40 mg a.s./L.

Description of key information

An acute 96-h toxicity test was conducted to estimate the toxicity of propineb to Rainbow Trout (Oncorhynchus mykiss). Ten fish per test concentration were exposed to nominal concentrations of 0.125, 0.25, 0.50, 1.00, 2.00 and 4.00 mg a.s./L. This study was conducted according to OECD 203. Based on nominal concentrations of propineb, the LC₅₀ (96 h) was determined to be 0.329 mg a.s./L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Remarks:

mortality

Effect concentration:

0.329 mg/L

Additional information

In addition, a study by Dogerloh (2004) as available. Test conditions met all validity criteria, given by the mentioned guidelines. The LC₅₀ (96 h) was determined to be >66.7 mg a.s./L.

A second additional study by Bruns (2011) is available. The test conditions met all validity criteria, given by the mentioned guidelines. The LC₅₀ (96 h) was determined to be 4.73 mg a.s./L.