(2S)-5-amino-2-[(aminoacetyl)amino]-5-oxopentanoic acid

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Radiolabelling:

no

Test temperature:

Column temperature: 35°C +/- 1°C

Details on study design: HPLC method:

EQUIPMENT
- Apparatus: Acquity UPLC system (Waters, Milford, MA, USA)
- Type, material and dimension of analytical (guard) column: Acquity UPLC HSS Cyano, 100 mm x 2.1 mm i.d., dp = 1.8 µm (Waters)
- Detection system: Acquity UPLC TUV detector (Waters)

MOBILE PHASES
- Type:
55/45 (v/v) methanol/buffer pH 5
55/45 (v/v) methanol/buffer pH 7
55/45 (v/v) methanol/buffer pH 9
- Experiments with additives carried out on separate columns: no
- pH: 5,7 and 9
- Flow: 0.4 mL/min
- Injection volume: 5 µL

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column

REFERENCE SUBSTANCES
- Identity: Acetanilide (CAS 103-84-4), Monuron (CAS 150-68-5), 2,5-Dichloroaniline (CAS 95-82-9), Naphthalene (CAS 91-20-3), Benzoic acid phenylester (CAS 93-99-2), Fenthion (CAS 55-38-9 ), Phenanthrene (CAS 85-01-8), 4,4’-DDT (CAS 50-29-3)

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 10.0 mg/L

REPETITIONS
- Number of determinations: 2

EVALUATION
- Determination of the log Koc value:
pH9: The equation of the regression line was: log k’ = 0.302 x log Koc – 0.895 (r = 0.98, n = 16)
pH7: The equation of the regression line was: log k’ = 0.315 x log Koc – 0.842 (r = 0.98, n = 16).
pH5: The equation of the regression line was: log k’ = 0.307 x log Koc – 0.873 (r = 0.98, n = 16).

Sample No.:

#3

Type:

log Koc

Value:

< 1.26

pH:

9

Sample No.:

#2

Type:

log Koc

Value:

< 1.26

pH:

7

Sample No.:

#1

Type:

log Koc

Value:

< 1.26

pH:

5

Details on results (HPLC method):

- Retention times of reference substances used for calibration [min]:

pH9:
Formamide (t0) 0.754/0.751
Acetanilide 0.943/0.939
Monuron 1.126/1.118
2,5-Dichloroaniline 1.242/1.232
Naphthalene 1.424/1.417
Benzoic acid phenylester 1.691/1.666
Fenthion 2.101/2.058
Phenanthrene 2.308/2.286
4,4’-DDT 4.875/4.804
- Average retention data for test substance pH 9 [min]: 0.515

pH7:
Formamide (t0) 0.733/0.730
Acetanilide 0.949/0.948
Monuron 1.164/1.160
2,5-Dichloroaniline 1.314/1.311
Naphthalene 1.530/1.528
Benzoic acid phenylester 1.815 /1.810
Fenthion 2.363/2.356
Phenanthrene 2.625/2.617
4,4’-DDT 6.122/6.083
- Average retention data for test substance pH7 [min]: 0.544


pH5:
Formamide (t0) 0.750/0.755
Acetanilide 0.961/0.958
Monuron 1.153/1.146
2,5-Dichloroaniline 1.281/1.276
Naphthalene 1.478/1.462
Benzoic acid phenylester 1.724/1.712
Fenthion 2.227/2.200
Phenanthrene 2.432/2.407
4,4’-DDT 5.469/5.382
- Average retention data for test substance pH5 [min]: 0.725

Conclusions:

The log Koc values of the test material at the environmental relevant pH 5, pH 7 and pH 9 were <1.26

Description of key information

The log Koc values of Glycyl-L-glutamine at the environmental relevant pH 5, pH 7 and pH 9 were <1.26