1,2,3-tris(2,3-epoxypropoxy)propane

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Species : Zebrafish, Danio rerio
Source : Gwangsu aquarium
Address : 24, Gajang-ro 534 beon-gil, Osan-si, Gyeonggi-do, Republic of Korea, 18104
Animal receipt : 2022. 04. 12.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

48 mg CaCO3/L at 0 h
46 mg CaCO3/L at 24 h
49 mg CaCO3/L at 48 h
45 mg CaCO3/L at 72 h

Test temperature:

21.6~21.8 °C

pH:

6.71~6.99

Dissolved oxygen:

7.63~8.81 mg/L(converted to air saturation value: 87.2~100.6 %)

Nominal and measured concentrations:

Nominal concentration (mg/L): 100.00
Geometric mean measured concentration (mg/L): 94.021

Details on test conditions:

The volume of test solution was prepared at 2 L in each test chamber(Ф210x270(mm)). The temperature[21~25 oC(initial temperature of control group±1 °C)], D.O. level(at least 60 % of the air-saturation value) and pH(6.0~8.5) were checked. Ten fish were placed in each chamber for 96 h under the semi-static test. Loading ratio of fish was not exceed 0.8 g fish/liter of solution. During the exposure period, the room was artificially illuminated with fluorescent lighting; 16 h light and 8 h dark cycle.

Reference substance (positive control):

yes

Remarks:

Copper(II) sulfate anhydrous

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

96 h LC50(mg/L) = 0.089

Validity criteria fulfilled:

yes

Conclusions:

The fish(Zebrafish, Danio rerio) was exposed to 100.00 mg/L treatment group of the test substance, KF EPIOL-PE311(GPGE), for 96 h under the semi-static test. A control group was also observed during this period. The acute toxicity assessment for fish was performed, and the LC50 was calculated.
As a result of the limit test, the geometric mean measured concentration at 100.00 mg/L were 94.021 mg/L.
These result were 94.0 % of the nominal concentration.
Therefore, all results were determined on the basis of the nominal concentration.
During an exposure period, there was no death in the limit test. Therefore, LC50 was reported as >100.00 mg/L at 48, 96 h after exposure. The highest concentration causing no mortality was reported as 100.00 mg/L at 48, 96 h after exposure, respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L